• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CytoSelect 96-well Phagocytosis Assay (Zymosan) Assay Kit

Scan QR to view Datasheet
Catalog # MBS168370
Unit / Price
  20 Assays  /  $380 +1 FREE 8GB USB
  96 Assays  /  $695 +1 FREE 8GB USB
  5x96 Assays  /  $2,780 +3 FREE 8GB USB
Product Name

CytoSelect 96-well Phagocytosis Assay (Zymosan), Assay Kit

Also Known As

CytoSelect 96-well Phagocytosis Assay (Zymosan)

Product Synonym Names
CytoSelect 96-well Phagocytosis Assay (Zymosan)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Preparation and Storage
Store all kit components at 4 degree C.
Product Note
Our Assay Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CytoSelect 96-well Phagocytosis Assay (Zymosan) assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Background/Introduction: In mammals, phagocytosis by phagocytes (e.g., macrophages, dendritic cells, and neutrophils) is essential for a variety of biological events, including tissue remodeling and the continuous clearance of dying cells. Furthermore, phagocytosis represents an early and crucial event in triggering host defenses against invading pathogens. Phagocytosis comprises a series of events, starting with the binding and recognition of particles by cell surface receptors, followed by the formation of actin-rich membrane extensions around the particle. Fusion of the membrane extensions results in phagosome formation, which precedes phagosome maturation into a phagolysosome. Pathogens inside the phagolysosome are destroyed by lowered pH, hydrolysis, and radical attack (Figure 1). These early events that are mediated by the innate immune system are critical for host survival. As a result of this process, pathogen-derived molecules can be presented at the cell surface (antigen presentation), allowing the induction of acquired immunity. Zymosan (Saccharomyces cerevisiae) is prepared from yeast cell wall and consists of protein-carbohydrate complexes. Zymosan is a commonly used pathogen in phagocytosis assays. Typically, engulfed Zymosan particles are manually counted (expressed as a phagocytosis index or engulfed particles per phagocyte). This manual counting method is quite cumbersome, time-consuming, and difficult when testing a large number of samples. CytoSelect™ 96-well Phagocytosis Assay (Zymosan) uses prelabeled Zymosan particles as a phagocytosis pathogen; however, it does not involve subjective manual counting of Zymosan particles inside cells. Instead external Zymosan particles are blocked before the colorimetric detection of engulfed particles (Figure 2). This format provides a quantitative, high-throughput method to accurately measure phagocytosis. The CytoSelect™ 96-well Phagocytosis Assay (Zymosan) provides a robust system for screening TLR ligands, phagocytosis activators or inhibitors. Each kit provides sufficient quantities to perform 96, 48, 24 tests in a 96, 48, 24-well plate, respectively.

Sample Manual Insert of MBS168370. Click to request current manual
1. Sansonetti, P. (2001) Semin. Immunol. 13:381-390.
2. Jutras I and Desjardins M. (2005) Annu Rev Cell Dev Biol. 21:511-27.
3. Janeway, C. A., Jr., and Medzhitov R. (2002) Annu. Rev. Immunol. 20:197-216.
4. Gordon, S. (2002) Cell. 111:927-930.
1. Fiorcari, S. et al. (2015). Lenalidomide interferes with tumor-promoting properties of nurse-like cells in chronic lymphocytic leukemia. Haematologica. 100:253-262.
2. Haselow, K. et al. (2013). Bile acids PKA-dependently induce a switch of the IL-10/IL-12 ratio and reduce proinflammatory capability of human macrophages. J. Leukoc. Biol. 94:1253-1264.
3. Pierce, L.M. et al. (2012). Effect of heavy metal tungsten alloy particles on oxidative product formation and phagocytosis by lung macrophages. Am. J. Respir. Crit. Care Med. 185:A4666.
4. Polancec, D.S.et al.(2012). Azithromycin drives in vitro GM-CSF/IL-4-induced differentiation of human blood monocytes toward dendritic-like cells with regulatory properties. J Leukoc Biol. 91:229-243.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000