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Enteropeptidase/Enterokinase Cleavage Kit Assay Kit

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Catalog # MBS841587
Unit / Price
  100 Assays  /  $285 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Enteropeptidase/Enterokinase Cleavage Kit, Assay Kit

Popular Item
Also Known As

Enteropeptidase / Enterokinase Cleavage Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Features & Benefits
Efficiently removes tags from recombinant fusion proteins containing accessible Enteropeptidase-specific recognition sequence.
Includes cleavage control protein (hPRL-Trx)
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Product Note
Our Assay Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Enteropeptidase/Enterokinase Cleavage Kit assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Information: Enteropeptidase/Enterokinase Cleavage Kit: Convenient tool to efficiently remove tags from recombinant fusion proteins containing accessible enteropeptidase-specific recognition sequence. Sufficient for cleaving at least 5 mg of target protein.

Background: Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence 'DDDDK' and cleaves after the lysine residue (K). The high specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion proteins containing the above recognition motif. MyBioSource's Enteropeptidase Cleavage Kit contains highly active light chain fragment of human Enteropeptidase. Our pure enzyme is an excellent tool to obtain a wild type protein sequence from a fusion protein, containing Enteropeptidase recognition sequence (DDDDK). This Enteropeptidase cleavage kit is sufficient for cleaving at least 5 mg of target protein. The residual enteropeptidase left in the reaction mix will not interfere with most of the downstream applications. Following cleavage of the target protein, Enteropeptidase can be removed using MyBioSource Hi-Bind Ni-QR Agarose beads.

Summary: Sufficient for cleaving at least 5 mg of target protein

Typical Testing Data/Standard Curve (for reference only) of Enteropeptidase/Enterokinase Cleavage Kit assay kit
Enteropeptidase/Enterokinase Cleavage Kit assay kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS841587. Click to request current manual
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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