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Phosphatidylcholine Assay Kit

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Catalog # MBS168279
Unit / Price
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  96 Assays  /  $505 +1 FREE 8GB USB
Product Name

Phosphatidylcholine, Assay Kit

Full Product Name

Phosphatidylcholine Assay Kit

Product Synonym Names
Phosphatidylcholine Assay Kit
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Preparation and Storage
Upon receipt, store the Phosphatidylcholine Standard, Fluorescence Probe, HRP, and Choline Oxidase at -20 degree C. The Fluorescence Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles. Store the Phospholipase D at -80 degree C. Store the remaining kit components at 4 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Phosphatidylcholine assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Phosphatidylcholine assay kit

Principle of the assay: Phosphatidylcholine Assay Kit measures the phosphatidylcholine present within serum, plasma, or tissue samples. The assay is based on the enzyme driven reaction that will detect phosphatidylcholine via phosphatidylcholine-specific phospholipase D enzyme and choline oxidase. First, phospholipase D hydrolyzes phosphatidylcholine into choline and phosphatidic acid. Choline is then oxidized by choline oxidase to produce hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific fluorescence probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of phosphatidylcholine standard within the 96-well microtiter plate format. Samples and standards are incubated for 60 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).

Background: Phospholipids are important structural lipids that are the major component of cell membranes and lipid bilayers. Phospholipids contain a hydrophilic head and a hydrophobic tail which give the molecules their unique characteristics. Most phospholipids contain one diglyceride, a phosphate group, and one choline group. Phosphatidylcholine is the foremost membrane phospholipid in eukaryotic cell membranes and is present in every cell in the body. It serves as a pool for many lipid messengers and is a source for bioactive lipids such as phosphatidic acid, diacylglycerol, lysophosphatidylcholine, and others. Regulation of phosphatidylcholine biosynthesis and metabolism is important to membrane structure maintenance and function. Phosphatidylcholine is the chief source of choline in the body, which itself and its derivative compounds form cell signaling molecules such as acetylcholine, platelet activating factor and sphingophosphorylcholine. As one of the most prevalent phospholipids in lipoproteins, phosphatidylcholine exerts considerable influence on lipid homeostasis. Since phosphatidylcholine comprises about 70% of the total phospholipids in plasma lipoproteins, it is very important to lipid homeostasis. Determining circulatory levels of phospholipids and lipoproteins is critical to the diagnosis of lipid transport disorders. Fatty acid residues from phosphatidylcholine are transferred via the enzyme lecithin cholesterol acyltransferase (LCAT) to cholesterol to create cholesterol esters. High levels of cholesterol and cholesterol esters (hypercholesterolemia) have been associated with cardiovascular disease such as atherosclerosis and heart disease. In addition, phosphatidylcholine synthesis is altered in many neurological, cardiovascular, and pulmonary disease states. Phosphatidylcholine Assay Kit is a simple fluorometric assay that measures the amount of phosphatidylcholine present in plasma or serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, phosphatidylcholine standards and unknown samples. Sample phosphatidylcholine concentrations are determined by comparison with a known phosphatidylcholine standard.

Phosphatidylcholine assay kit Testing Data #2 image
Sample Manual Insert of MBS168279. Click to request current manual
Product References and Citations for Phosphatidylcholine assay kit
1. Chapman, D., et al. (1967) Chem. Phys. Lipids 1: 445-475.
2. Hojjati, M.R., et al. (2006) J. Lipid Res. 47(3): 673-676.
3. Linsel-Nitchke, P., et al. (2005) Nature Reviews Drug Discovery 4: 193-205.
4. Ohta-Fukuyama, M. (1980) J. Biochem. 88: 197-203.
5. Phillips, G.B., et al. (1967) J. Lipid Res. 8: 676-681.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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