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Urea Assay Kit

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Catalog # MBS168363
Unit / Price
  192 Assays  /  $615 +1 FREE 8GB USB
Product Name

Urea, Assay Kit

Popular Item
Full Product Name

Urea Assay Kit

Product Synonym Names
Urea Assay Kit
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Samples
Serum, plasma and urine
Preparation and Storage
Upon receipt, prepare aliquots and store the Urea Standard and Urease at -20 degree C. Store the remaining kit components at 4 degree C until their expiration dates.
Product Note
Our Assay Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Urea assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Urea assay kit
Principle of the Assay: Urea Assay Kit measures urea levels within urine, serum, plasma, cell Iysates, or tissue homogenates. Samples are compared to a known concentration of urea standard within a 96-well microtiter plate format. Samples and standards are incubated for 10 minutes with the enzyme urease, which hydrolyzes urea to ammonia and CO2. The ammonia reacts further with a chromogen in alkali solution to produce a blue-green colored product. After 30 minutes, the plate is read with a standard 96-well spectrophotometric microplate reader at an optical density between 580 nm and 630 nm (Figure 2). Higher 00 values correlate with high urea concentrations. Sample urea concentrations are determined by comparison with the known urea standards. The standard curve is linear up to 50 mg/dL urea.

Background/Introduction: Urea, or carbamide, is the end product of protein nitrogen metabolism and is the primary vehicle for removing toxic ammonia from the body. Urea is synthesized in the liver from the ammonia produced from the catabolism of amino acids via the hepatic urea cycle. The conversion from ammonia to urea is regulated by N-acetylglutamate, which activates carbamoyl phosphate synthetase in the urea cycle. Urea is transported in the blood to the kidneys where it is excreted in the urine. In addition to its role as a carrier of waste nitrogen, urea also has a role in the countercurrent exchange system of the nephrons in which water and ions are re-absorbed from excreted urine. It is freely filtered by the glomeruli and partially passively resorbed as filtrate transverses the renal tubules. Urea reabsorption is inversely proportional to urine flow rate. Consequently, urea concentration depends upon protein intake, protein catabolism, and kidney function. Urea quantitation is one of the most widely applied tests for kidney function evaluation. The analysis of urea in serum, plasma and urine is an important clinical test for renal disease and dysfunction. The test is frequently tested in conjunction with creatinine determination for diagnosis of pre-renal, renal, and post renal uremia. Toxic urea levels are associated with renal, liver, or other system dysfunction. Pre-renal uremia relates to water depletion, increased protein catabolism, infection, hypovolemia, or cardiac decomposition. Glomerulonephritis, tubular necrosis, nephrosclerosis, chronic nephritis, and polycystic kidney are examples of renal uremia, while post renal uremia is predominantly urinary tract obstructions or leakage. Increased urea levels can also be linked to other disease states such as liver disease, diabetes, and congestive heart failure. High plasma urea levels are known as Azotemia. Decreased urea levels are associated with acute hepatic insufficiency or excess parenteral fluid therapy. Urea Assay Kit is based on the Berthelot reaction. Urea is first degraded into ammonia and carbon dioxide, which further reacts with an alkaline developer to produce a blue-green colored product that can be measured with a standard spectrophotometric plate reader at an optical density between 580-630 nm. Each kit provides sufficient reagents to perform up to 192 assays, including blanks, urea standards and unknown samples.

Testing Data of Urea assay kit
Urea assay kit Testing Data image
Testing Data #2 of Urea assay kit
Urea assay kit Testing Data #2 image
Testing Data #3 of Urea assay kit
Urea assay kit Testing Data #3 image
Sample Manual Insert of MBS168363. Click to request current manual
Protein Family
Product References and Citations for Urea assay kit
1. Friedman and Young. (2000) Effects of Disease on Clinical Laboratory Tests, 5th ed. AACC.
2. Sands, J.M. et al. (2009) Semin Nephrol. 29(3): 178-195.
3. Walker, V. (2009) Diabetes, Obes and Metab. 11(9): 823-835.
4. Young, D.S. et al. (1972) Clin. Chem. 18: 1041-1303.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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