View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Urinary Isoprostane Assay Kit Catalog # MBS480410 Unit / Price 1 Kit / $475 +1 FREE 8GB USB 5x1 Kit / $7,715 +9 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Urinary Isoprostane, Assay Kit Popular Item Full Product Name Urinary Isoprostane EIA Kit Product Synonym Names Urinary Isoprostane ELISA Kit Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Assay Type Competitive Preparation and Storage 1. Store this kit and its components at 4 degree C until use.2. Do not freeze. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of Urinary Isoprostane assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for Urinary Isoprostane assay kit Principle of the Assay: This kit is a competitive enzyme-linked immunoassay (ELISA) for determining levels of 15-isoprostane F2t (the best characterized isoprostane) in urine samples. Briefly, urine samples are mixed with an enhanced dilution buffer that essentially eliminates interference due to non-specific binding. The 15- isoprostane F2t in the samples or standards competes with 15-isoprostane F2t conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for 15-isoprostane F2t coated on the microplate. The HRP activity results in color development when substrate is added, with the intensity of the color proportional to the amount of 15-isoprostane F2t-HRP bound and inversely proportional to the amount of unconjugated 15-isoprostane F2t in the samples or standards.Background/Introduction: Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of 15-isoprostane F2t (also known as 8-epi-PGF2alphaor 8-iso- PGF2alpha) in urine samples. Levels of 15-isoprostane F2t in urine are useful for the non-invasive assessment of oxidant stress in vivo. 15-isoprostane F2t has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and plays a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis and carcinogenesis. Typical Testing Data/Standard Curve (for reference only) of Urinary Isoprostane assay kit TOP Sample Manual Insert of MBS480410. Product References and Citations for Urinary Isoprostane assay kit 1. Morrow, J., et. al., (1990) Anal. Biochem. 14:1-102. Morrow, J. D., et. al., (1990) Proc. Natl. Acad. Sci. U.S.A. 87:9383-93873. Morrow, et. al., (1997) Prog. Lipid Res. 36:1-214. Morrow, J., et. al., (1992)Proc. Natl. Acad. Sci USA 89:10721-107255. Wang, et. al., (1995) J. Pharm. Exp. Ther. 275:94-1006. Morrow, et. al., (1999) Analytical Biochem. 269: 326-3317. Roberts II, L.J., Morrow, J.D., (2000) Free Radic. Biol. Med. 28:505-5138. Morrow, J.D., Roberts II, L.J., Meth. Enz. 300: 3-12 TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP