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AT-III elisa kit :: Canine Anti-Thrombin III ELISA Kit

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Catalog # MBS743519
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Anti-Thrombin III (AT-III), ELISA Kit

Popular Item
Also Known As

Canine Anti-Thrombin III ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of AT3. No significant cross-reactivity or interference between AT3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AT3 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
25-500ng/mL
Sensitivity
1.0ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AT-III elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAT-IIIpurchase
MBS743519 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-Thrombin III (AT-III) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AT-III. The ELISA analytical biochemical technique of the MBS743519 kit is based on AT-III antibody-AT-III antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AT-III antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AT-III. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AT-III elisa kit
Intended Uses||This AT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine AT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: AT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AT3 antibody and an AT3-HRP conjugate. The assay sample and buffer are incubated together with AT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AT3 concentration since AT3 from samples and AT3-HRP conjugate compete for the anti-AT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by AT3 from the sample, fewer sites are left to bind AT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AT3 concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of AT-III elisa kit
AT-III elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS743519. Click to request current manual
NCBI/Uniprot data below describe general gene information for AT-III. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
Molecular Weight
52,004 Da
NCBI Official Full Name
antithrombin III
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
NCBI Official Symbol
Serpinc1  [Similar Products]
NCBI Official Synonym Symbols
At3; At-3; ATIII; AI114908
  [Similar Products]
NCBI Protein Information
antithrombin-III; serpin C1; anti-thrombin 3; serpin peptidase inhibitor, clade C, member 1; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
UniProt Protein Name
Antithrombin-III
UniProt Synonym Protein Names
Serpin C1
UniProt Gene Name
Serpinc1  [Similar Products]
UniProt Synonym Gene Names
At3; ATIII  [Similar Products]
UniProt Entry Name
ANT3_MOUSE
UniProt Comments for AT-III
SERPINC1: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D). AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin- binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Belongs to the serpin family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; protease binding; protease inhibitor activity

Biological Process: hemostasis; negative regulation of inflammatory response; negative regulation of peptidase activity; blood coagulation; response to nutrient
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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