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AT3G58490 elisa kit :: Human Sphingosine 1 Phosphate (Sphingosine 1 Phosphate) ELISA Kit

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Catalog # MBS766091
Unit / Price
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  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Sphingosine 1 Phosphate (Sphingosine 1 Phosphate) (AT3G58490), ELISA Kit

Popular Item
Full Product Name

Human Sphingosine 1 Phosphate (Sphingosine 1 Phosphate)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS766091 COA
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Sphingosine 1 Phosphate. No significant cross-reactivity or interference between Sphingosine 1 Phosphate and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
3.125-200ng/ml
Sensitivity
<1.875ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sphingosine 1 Phosphate were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sphingosine 1 Phosphate were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
4 degree C for 6 months
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AT3G58490 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for AT3G58490purchase
MBS766091 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sphingosine 1 Phosphate (Sphingosine 1 Phosphate) (AT3G58490) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AT3G58490. The ELISA analytical biochemical technique of the MBS766091 kit is based on AT3G58490 antibody-AT3G58490 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AT3G58490 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AT3G58490. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AT3G58490 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Sphingosine 1 Phosphate antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Sphingosine 1 Phosphate antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Sphingosine 1 Phosphate amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Sphingosine 1 Phosphate can be calculated.

Typical Testing Data/Standard Curve (for reference only) of AT3G58490 elisa kit
AT3G58490 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS766091. Click to request current manual
NCBI/Uniprot data below describe general gene information for AT3G58490. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
46,169 Da[Similar Products]
NCBI Official Full Name
sphingoid phosphate phosphatase 1
NCBI Official Symbol
AT3G58490  [Similar Products]
NCBI Protein Information
sphingoid phosphate phosphatase 1
UniProt Protein Name
Lipid phosphate phosphatase delta
UniProt Synonym Protein Names
Phosphatidic acid phosphatase delta; Sphingoid phosphate phosphatase 1; AtSSP1; Sphingosine-1-phosphate phosphatase; AtSPPASE
UniProt Gene Name
UniProt Synonym Gene Names
SPP1; AtLPPD; AtSSP1; AtSPPASE  [Similar Products]
UniProt Entry Name
LPPD_ARATH
UniProt Comments for AT3G58490
Functions as sphingoid long-chain base phosphate (LCBP) phosphatase. May play a role in the regulation of LCBP levels and be involved in stomatal responses through LCBP-mediated ABA signaling.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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