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ATG12 elisa kit :: Human ATG12 autophagy related 12 homolog (S. cerevisiae) ELISA Kit

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Catalog # MBS944212
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Typical Testing Data/Standard Curve (for reference only)
Product Name

ATG12 autophagy related 12 homolog (S. cerevisiae), ELISA Kit

Full Product Name

Human Ubiquitin-like protein ATG12, ATG12 ELISA Kit

Product Synonym Names
Human Ubiquitin-like protein ATG12 (ATG12) ELISA kit; APG12; APG12L; FBR93; HAPG12; APG12 autophagy 12-like; Apg12 (autophagy; yeast) homolog; ATG12 autophagy related 12 homolog (S. cerevisiae)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS944212 Testing Data
OMIM
609608
3D Structure
ModBase 3D Structure for O94817
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human ATG12. No significant cross-reactivity or interference between human ATG12 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
25 pg/ml -1600 pg/ml.
Sensitivity
The minimum detectable dose of human ATG12 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ATG12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ATG12purchase
MBS944212 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ATG12 autophagy related 12 homolog (S. cerevisiae) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATG12. The ELISA analytical biochemical technique of the MBS944212 kit is based on ATG12 antibody-ATG12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATG12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATG12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ATG12 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATG12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATG12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATG12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATG12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of ATG12 elisa kit
ATG12 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS944212. Click to request current manual
NCBI/Uniprot data below describe general gene information for ATG12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,113 Da
NCBI Official Full Name
ubiquitin-like protein ATG12 isoform 2
NCBI Official Synonym Full Names
autophagy related 12
NCBI Official Symbol
ATG12  [Similar Products]
NCBI Official Synonym Symbols
APG12; FBR93; APG12L; HAPG12
  [Similar Products]
NCBI Protein Information
ubiquitin-like protein ATG12; Apg12 (autophagy, yeast) homolog; ATG12 autophagy related 12 homolog
UniProt Protein Name
Ubiquitin-like protein ATG12
UniProt Synonym Protein Names
Autophagy-related protein 12; APG12-like
Protein Family
UniProt Gene Name
ATG12  [Similar Products]
UniProt Synonym Gene Names
APG12; APG12L; APG12-like  [Similar Products]
UniProt Entry Name
ATG12_HUMAN
NCBI Summary for ATG12
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
UniProt Comments for ATG12
ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-like modifier; Autophagy

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: pre-autophagosomal structure membrane

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: mitochondrion degradation; innate immune response; C-terminal protein lipidation; negative regulation of interferon type I production; autophagic vacuole formation; cellular response to nitrogen starvation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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