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ATG2A elisa kit :: Human Autophagy related protein 2 homolog A (ATG2A) ELISA Kit

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Catalog # MBS7229592
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Autophagy related protein 2 homolog A (ATG2A), ELISA Kit

Popular Item
Full Product Name

Human Autophagy related protein 2 homolog A (ATG2A) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ATG2A. No significant cross-reactivity or interference between ATG2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATG2A and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This ATG2A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATG2A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ATG2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forATG2Apurchase
MBS7229592 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Autophagy related protein 2 homolog A (ATG2A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATG2A. The ELISA analytical biochemical technique of the MBS7229592 kit is based on ATG2A antibody-ATG2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATG2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATG2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ATG2A elisa kit
Principle of the assay: ATG2A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATG2A antibody and an ATG2A-HRP conjugate. The assay sample and buffer are incubated together with ATG2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG2A concentration since ATG2A from samples and ATG2A-HRP conjugate compete for the anti-ATG2A antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG2A from the sample, fewer sites are left to bind ATG2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG2A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ATG2A elisa kit

Typical Testing Data/Standard Curve (for reference only) of ATG2A elisa kit
ATG2A elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7229592. Click to request current manual
NCBI/Uniprot data below describe general gene information for ATG2A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
13,576 Da
NCBI Official Full Name
autophagy-related protein 2 homolog A
NCBI Official Synonym Full Names
autophagy related 2A
NCBI Official Symbol
ATG2A  [Similar Products]
NCBI Protein Information
autophagy-related protein 2 homolog A; ATG2 autophagy related 2 homolog A
UniProt Protein Name
Autophagy-related protein 2 homolog A
Protein Family
UniProt Gene Name
ATG2A  [Similar Products]
UniProt Synonym Gene Names
KIAA0404  [Similar Products]
UniProt Entry Name
ATG2A_HUMAN
UniProt Comments for ATG2A
ATG2A: Belongs to the ATG2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: extrinsic to membrane; pre-autophagosomal structure membrane; lipid particle

Molecular Function: protein binding

Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with ATG2A elisa kit
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