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Atg7 recombinant protein

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Catalog # MBS153309
Unit / Price
  0.05 mg  /  $270 +1 FREE 8GB USB
SDS-PAGE
Product Name

ATG7, Recombinant Protein

Also Known As

ATG7 Recombinant Protein

Product Synonym Names
ATG7 Recombinant Protein: Agp7; Apg7l; Atg7l; Gm21553; 1810013K23Rik; autophagy related 7
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
698
Sequence
aa 450 - 698
3D Structure
ModBase 3D Structure for Q9D906
Purity/Purification
~95%
Form/Format
Liquid
Species
Mouse
Source
E Coli
Buffer
1X PBS containing 10% glycerol
Fusion Tag
Fusion Partner: C-terminal His-tag
Preparation and Storage
Store in working aliquots at -70 degree C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Other Notes
Small volumes of Atg7 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Atg7 recombinant protein
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog (4). In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (5). It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death (6).
Applications Tested/Suitable for Atg7 recombinant protein
ELISA (EIA), Western Blot (WB), MS
Application Notes for Atg7 recombinant protein
This recombinant protein can be used for WB, ELISA, MS and neutralization assays. For research use only.

SDS-PAGE analysis of recombinant ATG7 on Coomassie Blue-stained 4 - 12% SDS-PAGE gel.
Atg7 recombinant protein SDS-PAGE image
NCBI/Uniprot data below describe general gene information for Atg7. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30 kDa (Calculated)
NCBI Official Full Name
Autophagy-related 7 (yeast)
NCBI Official Synonym Full Names
autophagy related 7
NCBI Official Symbol
NCBI Official Synonym Symbols
Agp7; Apg7l; Atg7l; Gm21553; 1810013K23Rik
  [Similar Products]
NCBI Protein Information
ubiquitin-like modifier-activating enzyme ATG7; autophagy-related protein 7; ATG12-activating enzyme E1 ATG7; ubiquitin-activating enzyme E1-like protein
UniProt Protein Name
Ubiquitin-like modifier-activating enzyme ATG7
UniProt Synonym Protein Names
ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; mAGP7; Ubiquitin-activating enzyme E1-like protein
UniProt Gene Name
UniProt Synonym Gene Names
Apg7l; APG7-like; mAGP7  [Similar Products]
UniProt Entry Name
ATG7_MOUSE
UniProt Comments for Atg7
ATG7: Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Belongs to the ATG7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy

Cellular Component: cytoplasm; axoneme; cytosol

Molecular Function: protein binding; protein homodimerization activity; small protein activating enzyme activity; APG8 activating enzyme activity; APG12 activating enzyme activity; transcription factor binding

Biological Process: positive regulation of apoptosis; regulation of protein ubiquitination; macroautophagy; pyramidal neuron development; adult walking behavior; post-embryonic development; protein transport; cardiac muscle cell development; transport; protein amino acid lipidation; protein catabolic process; neurite development; positive regulation of autophagy; autophagic vacuole formation; amino acid metabolic process; mitochondrion organization and biogenesis; central nervous system neuron axonogenesis; organelle organization and biogenesis; cerebellar Purkinje cell layer development; C-terminal protein lipidation; cellular response to starvation; liver development; cellular response to nitrogen starvation; neurological system process; response to starvation; protein modification by small protein conjugation; positive regulation of protein catabolic process; positive regulation of protein modification process; mitochondrion degradation; autophagy; cerebral cortex development; positive regulation of macroautophagy; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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