• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ATP1b1 elisa kit :: Mouse ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2022937 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $270 +1 FREE 8GB USB
  48-Strip-Wells  /  $505 +1 FREE 8GB USB
  96-Strip-Wells  /  $685 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1), ELISA Kit

Also Known As

ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) ELISA Kit

Product Synonym Names
ATP1B; Sodium/potassium-dependent ATPase subunit beta-1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
301
OMIM
182330
3D Structure
ModBase 3D Structure for P05026
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1).
No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.071ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ATP1b1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forATP1b1purchase
MBS2022937 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATP1b1. The ELISA analytical biochemical technique of the MBS2022937 kit is based on ATP1b1 antibody-ATP1b1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATP1b1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATP1b1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ATP1b1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for ATP1b1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of ATP1b1 elisa kit
ATP1b1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2022937. Click to request current manual
NCBI/Uniprot data below describe general gene information for ATP1b1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34,893 Da
NCBI Official Full Name
ATPase, Na+/K+ transporting, beta 1 polypeptide, isoform CRA_b
NCBI Official Synonym Full Names
ATPase, Na+/K+ transporting, beta 1 polypeptide
NCBI Official Symbol
ATP1B1  [Similar Products]
NCBI Official Synonym Symbols
ATP1B
  [Similar Products]
NCBI Protein Information
sodium/potassium-transporting ATPase subunit beta-1
UniProt Protein Name
Sodium/potassium-transporting ATPase subunit beta-1
UniProt Synonym Protein Names
Sodium/potassium-dependent ATPase subunit beta-1
UniProt Gene Name
ATP1B1  [Similar Products]
UniProt Synonym Gene Names
ATP1B  [Similar Products]
UniProt Entry Name
AT1B1_HUMAN
NCBI Summary for ATP1b1
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010]
UniProt Comments for ATP1b1
ATP1B1: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: basolateral plasma membrane; apical plasma membrane; plasma membrane; caveola; sodium:potassium-exchanging ATPase complex; sarcolemma; vesicle

Molecular Function: protein binding; ATPase activator activity; sodium:potassium-exchanging ATPase activity; ATPase binding

Biological Process: cellular calcium ion homeostasis; cellular sodium ion homeostasis; protein stabilization; potassium ion import; positive regulation of ATPase activity; regulation of gene expression; response to hypoxia; blood coagulation; cell adhesion; cellular potassium ion homeostasis; leukocyte migration; transmembrane transport; cardiac muscle contraction

Disease: Hypertension, Essential
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000