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ATP2A1 blocking peptide :: SERCA1 Blocking Peptide

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Catalog # MBS822370
Unit / Price
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  1 mg  /  $160 +1 FREE 8GB USB
  5 mg  /  $305 +1 FREE 8GB USB
Product Name

SERCA1 (ATP2A1), Blocking Peptide

Popular Item
Full Product Name

SERCA1 Blocking Peptide

Product Synonym Names
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1; SR Ca(2+)-ATPase 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Antibody/Peptide Pairs
SERCA1 peptide (MBS822370) is used for blocking the activity of SERCA1 antibody (MBS821566)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 601003
3D Structure
ModBase 3D Structure for O14983
Host
Synthetic
Species Reactivity
Human, Mouse, Rat, Bovine, Rabbit
Purity/Purification
>85%
Form/Format
Lyophilized powder
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SERCA1. The exact sequence is proprietary.
Entrez Gene
487 (Human); 11937 (Mouse); 116601 (Rat)
Swiss Prot
O14983 (Human); Q8R429 (Mouse); Q64578 (Rat)
Quality Control
The quality of the peptide was evaluated by reversed - phase HPLC and by mass spectrometry.
Directions for Use
Blocking Peptide to the dil uted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Other Notes
Small volumes of ATP2A1 blocking peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ATP2A1 blocking peptide
The peptide is used to block Anti - SERCA1 Antibody reactivity.
Applications Tested/Suitable for ATP2A1 blocking peptide
Blocking

Western Blot (WB) of ATP2A1 blocking peptide
Western blot analysis of SERCA1 expression in A549 (A), mouse muscle (B), rat muscle (C) whole cell lysates.
ATP2A1 blocking peptide Western Blot (WB) (WB) image
Immunohistochemical of ATP2A1 blocking peptide
Immunohistochemical analysis of SERCA1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ATP2A1 blocking peptide Immunohistochemical image
NCBI/Uniprot data below describe general gene information for ATP2A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
95,199 Da
NCBI Official Full Name
sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform c
NCBI Official Synonym Full Names
ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
NCBI Official Symbol
ATP2A1  [Similar Products]
NCBI Official Synonym Symbols
ATP2A; SERCA1
  [Similar Products]
NCBI Protein Information
sarcoplasmic/endoplasmic reticulum calcium ATPase 1; calcium pump 1; SR Ca(2+)-ATPase 1; endoplasmic reticulum class 1/2 Ca(2+) ATPase; calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform
UniProt Protein Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
UniProt Synonym Protein Names
Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase
UniProt Gene Name
ATP2A1  [Similar Products]
UniProt Synonym Gene Names
SERCA1; SR Ca(2+)-ATPase 1  [Similar Products]
UniProt Entry Name
AT2A1_HUMAN
NCBI Summary for ATP2A1
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013]
UniProt Comments for ATP2A1
ATP2A1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Associated with sarcolipin (SLN) and phospholamban (PLN). Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression. Skeletal muscle, fast twitch muscle (type II) fibers. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.3.8; Transporter, ion channel; Endoplasmic reticulum; Hydrolase; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: I band; endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; membrane; sarcoplasmic reticulum; perinuclear region of cytoplasm; integral to membrane; ER-Golgi intermediate compartment

Molecular Function: protein binding; protein homodimerization activity; calcium-transporting ATPase activity; calcium ion binding; ATP binding

Biological Process: apoptotic mitochondrial changes; positive regulation of fast-twitch skeletal muscle contraction; maintenance of mitochondrion localization; metabolic process; reduction of endoplasmic reticulum calcium ion concentration; elevation of endoplasmic reticulum calcium ion concentration; calcium ion transport; regulation of striated muscle contraction; blood coagulation; transmembrane transport; elevation of mitochondrial calcium ion concentration; negative regulation of striated muscle contraction

Disease: Brody Myopathy
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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