• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ATP2A1 elisa kit :: Porcine Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS7238156
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1), ELISA Kit

Popular Item
Full Product Name

Porcine Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 601003
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ATP2A1. No significant cross-reactivity or interference between ATP2A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATP2A1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive (Quantitative)
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ATP2A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forATP2A1purchase
MBS7238156 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATP2A1. The ELISA analytical biochemical technique of the MBS7238156 kit is based on ATP2A1 antibody-ATP2A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATP2A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATP2A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ATP2A1 elisa kit
Intended Uses: This ATP2A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ATP2A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ATP2A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATP2A1 antibody and an ATP2A1-HRP conjugate. The assay sample and buffer are incubated together with ATP2A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP2A1 concentration since ATP2A1 from samples and ATP2A1-HRP conjugate compete for the anti-ATP2A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP2A1 from the sample, fewer sites are left to bind ATP2A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP2A1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ATP2A1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of ATP2A1 elisa kit
ATP2A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7238156. Click to request current manual
NCBI/Uniprot data below describe general gene information for ATP2A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
95,199 Da
NCBI Official Full Name
sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform a
NCBI Official Synonym Full Names
ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
NCBI Official Symbol
ATP2A1  [Similar Products]
NCBI Official Synonym Symbols
ATP2A; SERCA1
  [Similar Products]
NCBI Protein Information
sarcoplasmic/endoplasmic reticulum calcium ATPase 1; calcium pump 1; SR Ca(2+)-ATPase 1; endoplasmic reticulum class 1/2 Ca(2+) ATPase; calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform
UniProt Protein Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
UniProt Synonym Protein Names
Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase
UniProt Gene Name
ATP2A1  [Similar Products]
UniProt Synonym Gene Names
SERCA1; SR Ca(2+)-ATPase 1  [Similar Products]
UniProt Entry Name
AT2A1_HUMAN
NCBI Summary for ATP2A1
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013]
UniProt Comments for ATP2A1
ATP2A1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Associated with sarcolipin (SLN) and phospholamban (PLN). Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression. Skeletal muscle, fast twitch muscle (type II) fibers. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Transporter, ion channel; Hydrolase; Membrane protein, multi-pass; Membrane protein, integral; EC 3.6.3.8; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: I band; endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; membrane; sarcoplasmic reticulum; perinuclear region of cytoplasm; ER-Golgi intermediate compartment; integral to membrane

Molecular Function: protein binding; protein homodimerization activity; calcium-transporting ATPase activity; calcium ion binding; ATP binding

Biological Process: apoptotic mitochondrial changes; maintenance of mitochondrion localization; metabolic process; positive regulation of fast-twitch skeletal muscle contraction; elevation of endoplasmic reticulum calcium ion concentration; reduction of endoplasmic reticulum calcium ion concentration; calcium ion transport; regulation of striated muscle contraction; blood coagulation; transmembrane transport; negative regulation of striated muscle contraction; elevation of mitochondrial calcium ion concentration

Disease: Brody Myopathy
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000