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ATP6V0D2 elisa kit :: Human V type proton ATPase subunit d 2 (ATP6V0D2) ELISA Kit

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Catalog # MBS7232235
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

V type proton ATPase subunit d 2 (ATP6V0D2), ELISA Kit

Popular Item
Also Known As

Human V type proton ATPase subunit d 2 (ATP6V0D2) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ATP6V0D2. No significant cross-reactivity or interference between ATP6V0D2and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATP6V0D2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ATP6V0D2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forATP6V0D2purchase
MBS7232235 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the V type proton ATPase subunit d 2 (ATP6V0D2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATP6V0D2. The ELISA analytical biochemical technique of the MBS7232235 kit is based on ATP6V0D2 antibody-ATP6V0D2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATP6V0D2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATP6V0D2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ATP6V0D2 elisa kit
Intended Uses||This ATP6V0D2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATP6V0D2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ATP6V0D2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATP6V0D2 antibody and an ATP6V0D2-HRP conjugate. The assay sample and buffer are incubated together with ATP6V0D2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to theATP6V0D2 concentration since ATP6V0D2 from samples and ATP6V0D2-HRP conjugate compete for the anti-ATP6V0D2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP6V0D2 from the sample, fewer sites are left to bind ATP6V0D2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP6V0D2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ATP6V0D2 elisa kit

Typical Testing Data/Standard Curve (for reference only) of ATP6V0D2 elisa kit
ATP6V0D2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7232235. Click to request current manual
NCBI/Uniprot data below describe general gene information for ATP6V0D2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
40,426 Da
NCBI Official Full Name
V-type proton ATPase subunit d 2
NCBI Official Synonym Full Names
ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2
NCBI Official Symbol
ATP6V0D2  [Similar Products]
NCBI Official Synonym Symbols
VMA6; ATP6D2
  [Similar Products]
NCBI Protein Information
V-type proton ATPase subunit d 2; V-ATPase subunit d 2; vacuolar proton pump subunit d 2
UniProt Protein Name
V-type proton ATPase subunit d 2
UniProt Synonym Protein Names
Vacuolar proton pump subunit d 2
Protein Family
UniProt Gene Name
ATP6V0D2  [Similar Products]
UniProt Synonym Gene Names
V-ATPase subunit d 2  [Similar Products]
UniProt Entry Name
VA0D2_HUMAN
UniProt Comments for ATP6V0D2
ATP6V0D2: Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis. Belongs to the V-ATPase V0D/AC39 subunit family.

Protein type: Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14

Chromosomal Location of Human Ortholog: -

Cellular Component: phagocytic vesicle membrane; membrane; apical plasma membrane; early endosome; endosome membrane; vacuolar proton-transporting V-type ATPase complex

Molecular Function: protein binding; hydrogen ion transmembrane transporter activity

Biological Process: interaction with host; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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