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AURORA B recombinant protein

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Catalog # MBS515183
Unit / Price
  0.02 mg  /  $270 +1 FREE 8GB USB
  0.05 mg  /  $450 +1 FREE 8GB USB
SDS-PAGE
Product Name

AURORA B, Recombinant Protein

Full Product Name

AURORA B, Unactive recombinant protein

Product Synonym Names
AURKB; AIK2; AIM1; ARK2; AurB; IPL1; AIM-1; STK12
Product Synonym Gene Name
AURKB; AIK2; AIM1; ARK2; AurB; IPL1; AIM-1; STK12[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 17; NC_000017.10 (8108049..8113944, complement). Location: 17p13.1
OMIM
604970
3D Structure
ModBase 3D Structure for Q96GD4
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human AURORA B was expressed by baculovirus in Sf9 insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Other Notes
Small volumes of AURORA B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AURORA B recombinant protein
Recombinant full-length human AURORA B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: AURORA B is a member of the Aurora kinase family that associates with microtubules during chromosome movement and segregation. AURORA B localizes to the microtubules near kinetochores, specifically to the specialized microtubules called K-fibers (1). AURORA B inhibits the microtubule depolymerizing activity of mitotic centromere-associated kinesin (MCAK) by phosphorylating MCAK on Ser92 (2). This phosphorylation also regulates MCAK translocalization from kinetochores to the centromere. AURORA B has been identified as a target for the development of new anticancer agents since inhibition of AURORA B gives rise to the more pronounced antiproliferative phenotype.
Applications Tested/Suitable for AURORA B recombinant protein
Kinase Assay, Western Blot (WB)

NCBI/Uniprot data below describe general gene information for AURORA B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
~68 kDa
NCBI Official Full Name
Homo sapiens aurora kinase B (AURKB), transcript variant 1, mRNA
NCBI Official Synonym Full Names
aurora kinase B
NCBI Official Symbol
AURKB  [Similar Products]
NCBI Official Synonym Symbols
AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
  [Similar Products]
NCBI Protein Information
aurora kinase B; ARK-2; STK-1; aurora 1; aurora-1; aurora-B; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora-related kinase 2; serine/threonine kinase 12; aurora/IPL1-related kinase 2; serine/threonine-protein kinase 5; serine/threonine-protein kinase 12; serine/threonine-protein kinase aurora-B; protein phosphatase 1, regulatory subunit 48; aurora- and Ipl1-like midbody-associated protein 1
UniProt Protein Name
Aurora kinase B
UniProt Synonym Protein Names
Aurora 1; Aurora- and IPL1-like midbody-associated protein 1; AIM-1; Aurora/IPL1-related kinase 2; ARK-2; Aurora-related kinase 2; STK-1; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B
UniProt Gene Name
AURKB  [Similar Products]
UniProt Synonym Gene Names
AIK2; AIM1; AIRK2; ARK2; STK1; STK12; STK5; AIM-1; ARK-2  [Similar Products]
UniProt Entry Name
AURKB_HUMAN
NCBI Summary for AURORA B
This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2013]
UniProt Comments for AURORA B
Function: Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin, and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis. A positive feedback between GSG2 and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Ref.12 Ref.13 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.29 Ref.31 Ref.42 Ref.43

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Enzyme regulation: Activity is greatly increased when AURKB is within the CPC complex. In particular, AURKB-phosphorylated INCENP acts as an activator of AURKB. Positive feedback between GSG2 and AURKB contributes to CPC localization. Ref.20 Ref.23 Ref.25

Subunit structure: Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC. Associates with RACGAP1 during M phase. Interacts with CDCA1, EVI5, JTB, NDC80, PSMA3, SEPT1 and TACC1. Interacts with SPDYC; this interaction may be required for proper localization of active, Thr-232-phosphorylated AURKB form during prometaphase and metaphase. Interacts with p53/TP53. Interacts (via the middle kinase domain) with NOC2L (via the N- and C-terminus domains). Interacts with TTC28. Ref.12 Ref.17 Ref.20 Ref.21 Ref.23 Ref.25 Ref.26 Ref.27 Ref.28 Ref.30 Ref.34 Ref.38 Ref.39 Ref.43 Ref.44 Ref.45

Subcellular location: Nucleus. Chromosome. Chromosome › centromere. Cytoplasm › cytoskeleton › spindle. Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. Proper localization of the active, Thr-232-phosphorylated form during metaphase may be dependent upon interaction with SPDYC. Ref.12 Ref.13 Ref.18 Ref.20 Ref.28 Ref.38 Ref.43 Ref.45

Tissue specificity: High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. Ref.2 Ref.3

Induction: Expression is cell cycle-regulated, with a low in G1/S, an increase during G2 and M. Expression decreases again after M phase. Ref.20 Ref.23 Ref.25

Post-translational modification: The phosphorylation of Thr-232 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-232 phosphorylation is indispensable for the AURKB kinase activity.Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. Ref.32 Ref.36

Involvement in disease: Disruptive regulation of expression is a possible mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.Contains 1 protein kinase domain.

Sequence caution: The sequence AAH13300.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
Product References and Citations for AURORA B recombinant protein
1. Shannon, K B. et al: Chromosome dynamics: new light on Aurora B kinase function. Curr Biol. 2002 Jul 9;12(13):R458-60.
2. Andrews, P. D. et al: Aurora B regulates MCAK at the mitotic centromere. Dev. Cell 6: 253-268, 2004.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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