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AXIN elisa kit :: Rabbit Axis Inhibition Protein ELISA Kit

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Catalog # MBS728233
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
AXIN elisa kit
Product Name

Axis Inhibition Protein (AXIN), ELISA Kit

Full Product Name

Rabbit Axis Inhibition Protein ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 607864
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AXIN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAXINpurchase
MBS728233 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Axis Inhibition Protein (AXIN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AXIN. The ELISA analytical biochemical technique of the MBS728233 kit is based on AXIN antibody-AXIN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AXIN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AXIN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for AXIN elisa kit
NCBI/Uniprot data below describe general gene information for AXIN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
91,653 Da
NCBI Official Full Name
axin, partial
NCBI Official Synonym Full Names
axin 1
NCBI Official Symbol
AXIN1  [Similar Products]
NCBI Official Synonym Symbols
AXIN; PPP1R49
  [Similar Products]
NCBI Protein Information
axin-1; axis inhibitor 1; fused, mouse, homolog of; axis inhibition protein 1; protein phosphatase 1, regulatory subunit 49
UniProt Protein Name
Axin-1
UniProt Synonym Protein Names
Axis inhibition protein 1; hAxin
UniProt Gene Name
AXIN1  [Similar Products]
UniProt Synonym Gene Names
AXIN; hAxin  [Similar Products]
UniProt Entry Name
AXIN1_HUMAN
NCBI Summary for AXIN
This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2010]
UniProt Comments for AXIN
axin 1: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin-1 functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Wild-type axin 1 can induce apoptosis in hepatocellular and colorectal cancer cells. Is downregulated during progression of esophageal squamous cell carcinoma. Mutation of the axin-1 gene is associated with hepatocellular carcinoma, anaplastic thyroid cancer, medulloblastoma and colorectal cancer. May have a role in oncogenesis in Hodgkin lymphoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.

Protein type: Tumor suppressor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: Golgi apparatus; cytoplasmic microtubule; perinuclear region of cytoplasm; cytoplasm; cytoplasmic membrane-bound vesicle; postsynaptic density; cytoplasmic vesicle; cell cortex; beta-catenin destruction complex; cytosol; nucleus; lateral plasma membrane

Molecular Function: protein C-terminus binding; identical protein binding; protein homodimerization activity; protein self-association; p53 binding; beta-catenin binding; protein kinase binding; GTPase activator activity; protein binding; signal transducer activity; receptor signaling complex scaffold activity; enzyme binding; ubiquitin protein ligase binding; protein complex scaffold; SMAD binding; receptor binding

Biological Process: cell death; protein polyubiquitination; negative regulation of Wnt receptor signaling pathway; apoptosis; embryonic eye morphogenesis; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; negative regulation of protein metabolic process; nucleocytoplasmic transport; dorsal/ventral axis specification; Wnt receptor signaling pathway through beta-catenin; olfactory placode formation; cytoplasmic microtubule organization and biogenesis; activation of JNK activity; sensory perception of sound; positive regulation of ubiquitin-protein ligase activity; Wnt receptor signaling pathway involved in forebrain neuron fate commitment; protein catabolic process; protein homooligomerization; axial mesoderm formation; in utero embryonic development; positive regulation of transforming growth factor beta receptor signaling pathway; muscle cell development; negative regulation of fat cell differentiation; optic placode formation; positive regulation of peptidyl-serine phosphorylation; cellular protein complex assembly; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; forebrain anterior/posterior pattern formation; positive regulation of protein catabolic process; activation of protein kinase activity; positive regulation of protein amino acid phosphorylation; determination of left/right symmetry; positive regulation of GTPase activity

Disease: Caudal Duplication Anomaly; Hepatocellular Carcinoma
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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