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anti-b-Catenin (pY280) antibody :: Rabbit b-Catenin (pY280) Antibody

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Catalog # MBS462211
Unit / Price
  0.1 mg  /  $285 +1 FREE 8GB USB
Testing Data
Product Name

b-Catenin (pY280), Antibody

Full Product Name

Rabbit anti Phosph-beta-Catenin (Tyr280) antibody

Product Synonym Names
Rabbit anti-beta-Catenin (pY280)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 16; NC_007127.5 (7435502..7458779, complement)
3D Structure
ModBase 3D Structure for Q7ZU14
Host
Rabbit
Species Reactivity
Human, Rat, Mouse, Chicken, Canine
Specificity
This antibody recognizes ~88 kDa of human beta-Catenin protein. It also reacts with mouse and rat. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (lot specific)
Antigen Preparation
A synthetic peptide corresponding to the epitope EPYGL at the phosphorylation site Tyr280 of human beta-catenin.
Positive Control
HELA cell lysate
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Other Notes
Small volumes of anti-b-Catenin (pY280) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-b-Catenin (pY280) antibody
Beta-catenin is a cytosolic, 88 kDa, 781 amino acid protein belongs to the beta-catenin family. The N-terminus domain, containing the binding site and the phosphorylation sites. Beta-Catenin serves as a link between cytoskeleton actin and transmembrane cadherin (s). It is believed to contribute to tight cell-to-cell adhesion. It can enter the nucleus and interact with the TCF/LEF family of transcription factors, initiating gene expression. Normally, beta-catenin transcriptional activity is suppressed by a Ser/Thr kinase termed GSK3beta and/or Casein Kinase I (CK1). Kinases are constitutively active and phosphorylates beta-catenin at multiple sites, including S33 and S37, Y96, Y228, Y280 etc. Phosphorylation of beta-catenin targets the molecule for degradation via a ubiquination-mediated pathway. GSK3beta activity can be blocked by upstream signaling events such as Wnt-Frizzled interaction. This inhibits GSK3beta, allowing unphosphorylated beta-catenin to enter the nucleus and initiate gene activation. The phosphorylation of beta-catenin might contribute to tumorigenesis.
Applications Tested/Suitable for anti-b-Catenin (pY280) antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes for anti-b-Catenin (pY280) antibody
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml

NCBI/Uniprot data below describe general gene information for b-Catenin (pY280). It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
88 kDa
NCBI Official Full Name
b-catenin
NCBI Official Synonym Full Names
catenin (cadherin-associated protein), beta 1
NCBI Official Symbol
ctnnb1  [Similar Products]
NCBI Official Synonym Symbols
ctnnb; id:ibd2058; wu:fb73e10; wu:fi81c06; wu:fk25h01
  [Similar Products]
NCBI Protein Information
catenin beta-1; beta-catenin
UniProt Protein Name
Catenin (Cadherin-associated protein), beta 1
UniProt Gene Name
ctnnb1  [Similar Products]
UniProt Entry Name
Q7ZU14_DANRE
Product References and Citations for anti-b-Catenin (pY280) antibody
Park, C.S. et al. (2004) J. Biol. Chem. 279:19592.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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