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B2M elisa kit :: Dog beta-2-microglobulin ELISA Kit

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Catalog # MBS703912
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $575 +1 FREE 8GB USB
  96-Strip-Wells  /  $835 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

beta-2-microglobulin (B2M), ELISA Kit

Popular Item
Also Known As

Dog beta2-microglobulin, BMG/beta2-MG ELISA Kit

Product Synonym Names
Dog beta2-microglobulin; BMG/beta2-MG ELISA Kit; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS703912 Testing Data
Sequence Length
42
3D Structure
ModBase 3D Structure for P19341
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Dog B2M. No significant cross-reactivity or interference between Dog B2M and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.78 ug/ml-50 ug/ml
Sensitivity
0.2 ug/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Immunity
Protein Biological Process 3
Immunity
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of B2M elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forB2Mpurchase
MBS703912 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the beta-2-microglobulin (B2M) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing B2M. The ELISA analytical biochemical technique of the MBS703912 kit is based on B2M antibody-B2M antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect B2M antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, B2M. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
B2M elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta2-MG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta2-MG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta2-MG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta2-MG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of B2M elisa kit
B2M elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS703912. Click to request current manual
NCBI/Uniprot data below describe general gene information for B2M. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
4,842 Da
NCBI Official Full Name
Beta-2-microglobulin, partial
UniProt Protein Name
Beta-2-microglobulin
Protein Family
UniProt Gene Name
UniProt Entry Name
B2MG_CANFA
UniProt Comments for B2M
Function: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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