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B7 H4 elisa kit :: Human Costimulatory molecules B7H4 ELISA Kit

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Catalog # MBS751659
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Costimulatory molecules B7H4 (B7 H4), ELISA Kit

Popular Item
Also Known As

Human Costimulatory molecules B7H4 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
608162
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of B7H4. No significant cross-reactivity or interference between B7H4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between B7H4 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This B7H4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human B7H4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of B7 H4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forB7 H4purchase
MBS751659 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Costimulatory molecules B7H4 (B7 H4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing B7 H4. The ELISA analytical biochemical technique of the MBS751659 kit is based on B7 H4 antibody-B7 H4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect B7 H4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, B7 H4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
B7 H4 elisa kit
Priciple of the assay: B7H4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-B7H4 antibody and an B7H4-HRP conjugate. The assay sample and buffer are incubated together with B7H4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the B7H4 concentration since B7H4 from samples and B7H4-HRP conjugate compete for the anti-B7H4 antibody binding site. Since the number of sites is limited, as more sites are occupied by B7H4 from the sample, fewer sites are left to bind B7H4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The B7H4 concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of B7 H4 elisa kit
B7 H4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS751659. Click to request current manual
NCBI/Uniprot data below describe general gene information for B7 H4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,743 Da
NCBI Official Full Name
B7-H4
NCBI Official Synonym Full Names
V-set domain containing T cell activation inhibitor 1
NCBI Official Symbol
VTCN1  [Similar Products]
NCBI Official Synonym Symbols
B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
  [Similar Products]
NCBI Protein Information
V-set domain-containing T-cell activation inhibitor 1; B7 family member, H4; B7 superfamily member 1; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4
UniProt Protein Name
V-set domain-containing T-cell activation inhibitor 1
UniProt Synonym Protein Names
B7h.5; Immune costimulatory protein B7-H4; Protein B7S1; T-cell costimulatory molecule B7x
UniProt Gene Name
VTCN1  [Similar Products]
UniProt Synonym Gene Names
B7H4; UNQ659/PRO1291  [Similar Products]
UniProt Entry Name
VTCN1_HUMAN
NCBI Summary for B7 H4
This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Comments for B7 H4
VTCN1: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: receptor binding

Biological Process: response to protozoan; immune system process; positive regulation of T cell proliferation; negative regulation of T cell activation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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