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BAAT elisa kit :: Mouse Bile Acid-CoA:Amino Acid N-Acyltransferase (BAAT) ELISA Kit

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Catalog # MBS9347865
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
BAAT elisa kit
Product Name

Bile Acid-CoA:Amino Acid N-Acyltransferase (BAAT), ELISA Kit

Popular Item
Also Known As

Mouse Bile Acid-CoA:Amino Acid N-Acyltransferase (BAAT) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
602938
Species Reactivity
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Assay Type
Quantitative Sandwich
Detection Range
0.625 ng/ml - 20 ng/ml
Sensitivity
0.1 ng/ml.
Intra-assay Precision
Intra-assay CV (%) is less than 15%
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BAAT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forBAATpurchase
MBS9347865 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bile Acid-CoA:Amino Acid N-Acyltransferase (BAAT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BAAT. The ELISA analytical biochemical technique of the MBS9347865 kit is based on BAAT antibody-BAAT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BAAT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BAAT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BAAT elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of BAAT (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS9347865. Click to request current manual
NCBI/Uniprot data below describe general gene information for BAAT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,299 Da
NCBI Official Full Name
bile acid-CoA:amino acid N-acyltransferase
NCBI Official Synonym Full Names
bile acid-CoA:amino acid N-acyltransferase
NCBI Official Symbol
NCBI Official Synonym Symbols
BAT; BACAT
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NCBI Protein Information
bile acid-CoA:amino acid N-acyltransferase
UniProt Protein Name
Bile acid-CoA:amino acid N-acyltransferase
UniProt Synonym Protein Names
Glycine N-choloyltransferase; Long-chain fatty-acyl-CoA hydrolase
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
BAAT_HUMAN
NCBI Summary for BAAT
The protein encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for BAAT
BAAT: Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs. Defects in BAAT are involved in familial hypercholanemia (FHCA). FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. Belongs to the C/M/P thioester hydrolase family.

Protein type: Lipid Metabolism - unsaturated fatty acid biosynthesis; EC 3.1.2.2; Hydrolase; Lipid Metabolism - primary bile acid biosynthesis; Transferase; EC 2.3.1.65; Other Amino Acids Metabolism - taurine and hypotaurine

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: cytosol; peroxisomal matrix; peroxisome

Molecular Function: glycine N-choloyltransferase activity; N-acyltransferase activity; palmitoyl-CoA hydrolase activity; protein binding; receptor binding

Biological Process: acyl-CoA metabolic process; bile acid and bile salt transport; bile acid biosynthetic process; bile acid metabolic process; fatty acid metabolic process; glycine metabolic process; liver development; organ regeneration; taurine metabolic process

Disease: Hypercholanemia, Familial
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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