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BAD elisa kit :: Rat BAD ELISA Kit

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Catalog # MBS2507517
Unit / Price
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  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name


Popular Item
Full Product Name

Rat BAD (BCL-2 Associated Death Promoter) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q92934
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of Rat BAD. No significant cross-reactivity or interference between Rat BAD and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat BAD and all the analogues, therefore, cross reaction may still exist.
Serum, Plasma, Biological Fluids
Assay Type
Detection Range
Min: 18.75pg/mL; Max: 2000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BAD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for BADpurchase
MBS2507517 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BAD, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BAD. The ELISA analytical biochemical technique of the MBS2507517 kit is based on BAD antibody-BAD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BAD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BAD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BAD elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BAD in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BAD. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat BAD and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BAD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat BAD. You can calculate the concentration of Rat BAD in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of BAD elisa kit
BAD elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2507517. Click to request current manual
NCBI/Uniprot data below describe general gene information for BAD. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,392 Da
NCBI Official Full Name
bcl2 antagonist of cell death
NCBI Official Synonym Full Names
BCL2-associated agonist of cell death
NCBI Official Symbol
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
bcl2-associated agonist of cell death; BCL-X/BCL-2 binding protein; BCL2-antagonist of cell death protein; BCL2-binding component 6; BCL2-binding protein; bcl-2-binding component 6; bcl-2-like protein 8; bcl-XL/Bcl-2-associated death promoter; bcl2 antagonist of cell death; bcl2-L-8
UniProt Protein Name
Bcl2-associated agonist of cell death
UniProt Synonym Protein Names
Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl2-L-8; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death
UniProt Gene Name
UniProt Synonym Gene Names
BBC6; BCL2L8; BAD; Bcl2-L-8  [Similar Products]
UniProt Entry Name
NCBI Summary for BAD
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]
UniProt Comments for BAD
BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; protein kinase binding; lipid binding; protein phosphatase 2B binding

Biological Process: response to oleate; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; positive regulation of proteolysis; response to glucocorticoid stimulus; positive regulation of caspase activity; glucose homeostasis; cellular process regulating host cell cycle in response to virus; positive regulation of apoptosis by virus; response to estradiol stimulus; response to glucose stimulus; pore complex biogenesis; positive regulation of autophagy; positive regulation of glucokinase activity; caspase activation; response to drug; epidermal growth factor receptor signaling pathway; release of cytochrome c from mitochondria; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; positive regulation of insulin secretion; ADP metabolic process; response to testosterone stimulus; response to amino acid stimulus; ATP metabolic process; glucose catabolic process; response to ethanol; response to hydrogen peroxide; positive regulation of T cell differentiation; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; innate immune response; positive regulation of B cell differentiation; regulation of mitochondrial membrane permeability; response to calcium ion; response to progesterone stimulus; positive regulation of epithelial cell proliferation
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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