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BCL2L1 elisa kit :: Mouse Bcl-2-like protein 1 (BCL2L1) ELISA Kit

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Catalog # MBS7216022
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Bcl-2-like protein 1 (BCL2L1), ELISA Kit

Popular Item
Full Product Name

Mouse Bcl-2-like protein 1 (BCL2L1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
600039
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of BCL2L1. No significant cross-reactivity or interference between BCL2L1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BCL2L1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BCL2L1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for BCL2L1purchase
MBS7216022 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bcl-2-like protein 1 (BCL2L1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BCL2L1. The ELISA analytical biochemical technique of the MBS7216022 kit is based on BCL2L1 antibody-BCL2L1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BCL2L1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BCL2L1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BCL2L1 elisa kit
Intended Uses: This BCL2L1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BCL2L1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: BCL2L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BCL2L1 antibody and an BCL2L1-HRP conjugate. The assay sample and buffer are incubated together with BCL2L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL2L1 concentration since BCL2L1 from samples and BCL2L1-HRP conjugate compete for the anti-BCL2L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL2L1 from the sample, fewer sites are left to bind BCL2L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL2L1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for BCL2L1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of BCL2L1 elisa kit
BCL2L1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7216022. Click to request current manual
NCBI/Uniprot data below describe general gene information for BCL2L1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,290 Da
NCBI Official Full Name
bcl-2-like protein 1 isoform 1
NCBI Official Synonym Full Names
BCL2-like 1
NCBI Official Symbol
BCL2L1  [Similar Products]
NCBI Official Synonym Symbols
BCLX; BCL2L; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; PPP1R52; BCL-XL/S
  [Similar Products]
NCBI Protein Information
bcl-2-like protein 1; apoptosis regulator Bcl-X; protein phosphatase 1, regulatory subunit 52
UniProt Protein Name
Bcl-2-like protein 1
UniProt Synonym Protein Names
Apoptosis regulator Bcl-X
Protein Family
UniProt Gene Name
BCL2L1  [Similar Products]
UniProt Synonym Gene Names
BCL2L; BCLX; Bcl2-L-1  [Similar Products]
UniProt Entry Name
B2CL1_HUMAN
NCBI Summary for BCL2L1
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008]
UniProt Comments for BCL2L1
Bcl-xL: an antiapoptotic member of the Bcl-2 family. Located at the outer mitochondrial membrane and regulates outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are potent inducers of cell apoptosis. Two alternatively spliced isoforms have been reported.

Protein type: Membrane protein, integral; Mitochondrial; Autophagy; Apoptosis

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: centrosome; nuclear membrane; mitochondrion; synaptic vesicle membrane; integral to membrane; cytosol; mitochondrial outer membrane; mitochondrial matrix; mitochondrial inner membrane; cytoplasm; nucleolus; nucleus; cell junction

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; BH3 domain binding; protein kinase binding

Biological Process: negative regulation of autophagy; apoptosis; cytokinesis; cellular process regulating host cell cycle in response to virus; germ cell development; apoptotic mitochondrial changes; regulation of mitochondrial membrane potential; ovarian follicle development; positive regulation of cell proliferation; negative regulation of neuron apoptosis; release of cytochrome c from mitochondria; in utero embryonic development; mitotic cell cycle checkpoint; suppression by virus of host apoptosis; male gonad development; endocytosis; cell proliferation; neuron apoptosis; fertilization; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; response to cycloheximide; innate immune response; spermatogenesis; regulation of mitochondrial membrane permeability; negative regulation of apoptosis; growth
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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