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bCMO1 recombinant protein :: Beta-Carotene-15,15'-Monooxygenase 1 Recombinant Protein

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Catalog # MBS2033403 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $140 +1 FREE 8GB USB
  0.05 mg  /  $265 +1 FREE 8GB USB
  0.1 mg  /  $410 +1 FREE 8GB USB
  0.2 mg  /  $505 +1 FREE 8GB USB
  0.5 mg  /  $980 +1 FREE 8GB USB
  1 mg  /  $1,445 +1 FREE 8GB USB
Product Name

Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1), Recombinant Protein

Popular Item
Full Product Name

Recombinant Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1)

Product Synonym Names
BCO1; BCDO; BCDO1; BCMO; Beta-carotene dioxygenase 1; Beta-carotene oxygenase 1; Beta,beta-carotene 15,15'-monooxygenase
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2033403 COA
Sequence
GRNRK EQLEPVRAKV TGKIPAWLQG TLLRNGPGMH TVGESRYNHW FDGLALLHSF TIRDGEVYYR SKYLRSDTYN TNIEANRIVV SEFGTMAYPD PCKNIFSKAF SYLSHTIPDF TDNCLINIMK CGEDFYATSE TNYIRKINPQ TLETLEKVDY RKYVAVNLAT SHPHYDEAGN VLNMGTSIVE KGKTKYVIFK IPATVP
OMIM
115300
Host
E Coli
Purity/Purification
>99%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Source
Prokaryotic expression.
Residues
Gly6~Pro206
Tags
N-terminal His-Tag
Subcellular Location
Cytosol.
Traits
Freeze-dried powder
Predicted isoelectric point
8.9
Predicted Molecular Mass
26.6kDa
Accurate Molecular Mass
27kDa as determined by SDS-PAGE reducing conditions.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of bCMO1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for bCMO1 recombinant protein
SDS-PAGE, WB, ELISA, IP, CoIP, ReporterAssays, Purification, Amine Reactive Labeling.
(May be suitable for use in other assays to be determined by the end user.)

Sequence Information of bCMO1 recombinant protein
bCMO1 recombinant protein Sequence Information image
NCBI/Uniprot data below describe general gene information for bCMO1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
62,637 Da
NCBI Official Full Name
beta,beta-carotene 15,15'-dioxygenase
NCBI Official Synonym Full Names
beta-carotene oxygenase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
BCO; BCDO; BCMO; BCDO1; BCMO1
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NCBI Protein Information
beta,beta-carotene 15,15'-dioxygenase
UniProt Protein Name
Beta,beta-carotene 15,15'-dioxygenase
UniProt Synonym Protein Names
Beta-carotene dioxygenase 1; Beta-carotene oxygenase 1
UniProt Gene Name
UniProt Entry Name
BCDO1_HUMAN
NCBI Summary for bCMO1
Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. [provided by RefSeq, Jul 2008]
UniProt Comments for bCMO1
BCMO1: Symmetrically cleaves beta-carotene into two molecules of retinal. The reaction proceeds in three stages, epoxidation of the 15,15'-double bond, hydration of the double bond leading to ring opening, and oxidative cleavage of the diol formed. Defects in BCMO1 are the cause of autosomal dominant hypercarotenemia and vitamin A deficiency (ADHVAD). Vitamin A is essential for normal embryonic development as well as normal physiological functions in children and adults. Hypercarotenemia is characterized by an excess carotene in the serum, but unlike excess vitamin A, carotene is non-toxic. So far, only a few cases of excess vitamin A have been reported. Individuals were thought to be vitamin A deficient due to an impairment in the conversion of carotenoids to retinal in the intestine. Belongs to the carotenoid oxygenase family.

Protein type: EC 1.14.99.36; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 16q23.2

Cellular Component: cytosol

Molecular Function: beta-carotene 15,15'-monooxygenase activity; metal ion binding

Biological Process: fat-soluble vitamin metabolic process; phototransduction, visible light; retinal metabolic process; retinoid metabolic process; retinol metabolic process; vitamin A biosynthetic process; vitamin metabolic process

Disease: Hypercarotenemia And Vitamin A Deficiency, Autosomal Dominant
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withbCMO1 recombinant protein
 Reference Product  PubMed Publications
 ISX recombinant protein  >2 publications with bCMO1 and ISX
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