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BDNF elisa kit :: Human BDNF ELISA Kit

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Catalog # MBS355324
Unit / Price
  96 Tests  /  $375 +1 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

BDNF, ELISA Kit

Popular Item
Full Product Name

BDNF Human ELISA Kit

Product Synonym Names
Human BDNF
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Samples
human serum, plasma, body fluids, tissue lysates or cell culture supernatants
Assay Type
Quantitative Sandwich
Detection Range
31.2 pg/ml-2000 pg/ml
Sensitivity
< 2 pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BDNF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forBDNFpurchase
MBS355324 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BDNF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BDNF. The ELISA analytical biochemical technique of the MBS355324 kit is based on BDNF antibody-BDNF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BDNF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BDNF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BDNF elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BDNF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BDNF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BDNF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BDNF can be calculated.

Background: Brain-derived neurotrophic factor, also known as BDNF, is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. BDNF is a member of the "neurotrophin" family of growth factors, which are related to the NGF. It acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. In the brain, it is active in the hippocampus, cortex, and basal forebrain—areas vital to learning, memory, and higher thinking. Post mortem analysis has shown lowered levels of BDNF in the brain tissues of people with Alzheimer's disease.
Applications Tested/Suitable for BDNF elisa kit
Quantitative sELISA (EIA)
Application Notes for BDNF elisa kit
For quantitative detection of BDNF in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Typical Testing Data/Standard Curve (for reference only) of BDNF elisa kit
BDNF elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS355324. Click to request current manual
NCBI/Uniprot data below describe general gene information for BDNF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI Official Full Name
BDNF, partial
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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