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BECN1 elisa kit :: Rat Beclin 1 ELISA Kit

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Catalog # MBS2533549
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Beclin 1 (BECN1), ELISA Kit

Popular Item
Also Known As

Rat BECN1 (Beclin 1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
450
OMIM
604378
Species Reactivity
Specificity
This kit recognizes natural and recombinantRatBECN1. No significant cross-reactivity or interference between RatBECN1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BECN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forBECN1purchase
MBS2533549 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Beclin 1 (BECN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BECN1. The ELISA analytical biochemical technique of the MBS2533549 kit is based on BECN1 antibody-BECN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BECN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BECN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BECN1 elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatBECN1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BECN1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BECN1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BECN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BECN1.You can calculate the concentration of BECN1in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of BECN1 elisa kit
BECN1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2533549. Click to request current manual
NCBI/Uniprot data below describe general gene information for BECN1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,896 Da
NCBI Official Full Name
beclin 1
NCBI Official Synonym Full Names
beclin 1, autophagy related
NCBI Official Symbol
BECN1  [Similar Products]
NCBI Official Synonym Symbols
ATG6; VPS30; beclin1
  [Similar Products]
NCBI Protein Information
beclin-1
UniProt Protein Name
Beclin-1
UniProt Synonym Protein Names
Coiled-coil myosin-like BCL2-interacting protein; Protein GT197
Protein Family
UniProt Gene Name
BECN1  [Similar Products]
UniProt Synonym Gene Names
GT197  [Similar Products]
UniProt Entry Name
BECN1_HUMAN
NCBI Summary for BECN1
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
UniProt Comments for BECN1
beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

Protein type: Membrane protein, peripheral; Autophagy; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: autophagic vacuole; cytoplasmic membrane-bound vesicle; cytosol; dendrite; endoplasmic reticulum; endoplasmic reticulum membrane; endosome; endosome membrane; extrinsic to membrane; mitochondrial membrane; mitochondrion; nucleus; phagocytic vesicle; trans-Golgi network

Molecular Function: phosphoinositide 3-kinase binding; protein binding; ubiquitin protein ligase binding

Biological Process: apoptosis; autophagic vacuole formation; autophagy; beta-amyloid metabolic process; cellular defense response; cellular response to glucose starvation; cellular response to nitrogen starvation; CVT pathway; cytokinesis; defense response to virus; engulfment of apoptotic cell; late endosome to vacuole transport; lysosome organization and biogenesis; macroautophagy; mitotic metaphase plate congression; negative regulation of apoptosis; negative regulation of cell proliferation; neuron development; positive regulation of autophagy; positive regulation of phosphoinositide 3-kinase cascade; receptor catabolic process; regulation of catalytic activity; regulation of cytokinesis; response to drug; response to hypoxia; response to vitamin E; viral reproduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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