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BH2 elisa kit :: Rat Dihydrobiopterin ELISA Kit

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Catalog # MBS2533583
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Dihydrobiopterin (BH2), ELISA Kit

Popular Item
Also Known As

Rat BH2 (Dihydrobiopterin) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS2533583 COA
MBS2533583 Testing Data
Species Reactivity
Specificity
This kit recognizes natural and recombinantRatBH2. No significant cross-reactivity or interference between RatBH2 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Competitive
Detection Range
31.25-2000pg/mL
Sensitivity
Min: 18.75pg/mL; Max: 2000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BH2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forBH2purchase
MBS2533583 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dihydrobiopterin (BH2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BH2. The ELISA analytical biochemical technique of the MBS2533583 kit is based on BH2 antibody-BH2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BH2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BH2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BH2 elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatBH2concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with BH2. During the reaction,BH2 in the sample or standard competes with a fixed amount ofBH2on the solid phase supporterfor sites on the Biotinylated Detection Ab specific toBH2. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration ofBH2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of BH2 elisa kit
BH2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2533583. Click to request current manual
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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