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BIP.Ab elisa kit :: Human Anti immunobulin Binding Protein antibody ELISA Kit

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Catalog # MBS729359
Unit / Price
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  48-Strip-Wells-(Competitive)  /  $440 +1 FREE 8GB USB
  48-Strip-Wells-(Sandwich)  /  $440 +1 FREE 8GB USB
  96-Strip-Wells-(Competitive)  /  $640 +1 FREE 8GB USB
  96-Strip-Wells-(Sandwich)  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells-(Competitive)  /  $2,895 +3 FREE 8GB USB
  5x96-Strip-Wells-(Sandwich)  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells-(Competitive)  /  $5,415 +6 FREE 8GB USB
  10x96-Strip-Wells-(Sandwich)  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Anti immunobulin Binding Protein antibody (BIP.Ab), ELISA Kit

Full Product Name

Human Anti immunobulin Binding Protein antibody ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
138120
3D Structure
ModBase 3D Structure for P11021
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
0.5-10ug/mL
Sensitivity
0.1ug/mL
Intended Uses
This BIP.Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BIP.Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BIP.Ab elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for BIP.Abpurchase
MBS729359 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti immunobulin Binding Protein antibody (BIP.Ab) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BIP.Ab. The ELISA analytical biochemical technique of the MBS729359 kit is based on BIP.Ab antibody-BIP.Ab antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BIP.Ab antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BIP.Ab. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BIP.Ab elisa kit
Principle of the Assay: BIP.Ab ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BIP.Ab. Standards or samples are then added to the microtiter plate wells and BIP.Ab if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BIP.Ab present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for BIP.Ab are added to each well to "sandwich" the BIP.Ab immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BIP.Ab and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BIP.Ab concentration in each sample is interpolated from this standard curve.
Product Categories/Family for BIP.Ab elisa kit

Typical Testing Data/Standard Curve (for reference only) of BIP.Ab elisa kit
BIP.Ab elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS729359. Click to request current manual
NCBI/Uniprot data below describe general gene information for BIP.Ab. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
72,333 Da
NCBI Official Full Name
BiP
NCBI Official Synonym Full Names
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
NCBI Official Symbol
HSPA5  [Similar Products]
NCBI Official Synonym Symbols
BIP; MIF2; GRP78; HEL-S-89n
  [Similar Products]
NCBI Protein Information
78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; epididymis secretory sperm binding protein Li 89n; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
UniProt Protein Name
78 kDa glucose-regulated protein
UniProt Synonym Protein Names
Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP
UniProt Gene Name
HSPA5  [Similar Products]
UniProt Synonym Gene Names
GRP78; GRP-78; BiP  [Similar Products]
UniProt Entry Name
GRP78_HUMAN
NCBI Summary for BIP.Ab
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
UniProt Comments for BIP.Ab
GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: signalosome; endoplasmic reticulum membrane; cell surface; focal adhesion; smooth endoplasmic reticulum; mitochondrion; endoplasmic reticulum lumen; endoplasmic reticulum; ER-Golgi intermediate compartment; membrane; melanosome; plasma membrane; integral to endoplasmic reticulum membrane; midbody; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; chaperone binding; ubiquitin protein ligase binding; unfolded protein binding; ATPase activity; ribosome binding; calcium ion binding; misfolded protein binding; glycoprotein binding; ATP binding

Biological Process: platelet activation; ER-associated protein catabolic process; cerebellum structural organization; unfolded protein response; cerebellar Purkinje cell layer development; cellular response to glucose starvation; unfolded protein response, activation of signaling protein activity; platelet degranulation; cellular protein metabolic process; substantia nigra development; positive regulation of protein ubiquitination; blood coagulation; negative regulation of transforming growth factor beta receptor signaling pathway; ER overload response; negative regulation of apoptosis; positive regulation of cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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