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BIRC2 elisa kit :: Human Baculoviral IAP repeat containing protein 2 (BIRC2) ELISA Kit

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Catalog # MBS7217561
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Baculoviral IAP repeat containing protein 2 (BIRC2), ELISA Kit

Popular Item
Full Product Name

Human Baculoviral IAP repeat containing protein 2 (BIRC2) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
601712
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of BIRC2. No significant cross-reactivity or interference between BIRC2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BIRC2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BIRC2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for BIRC2purchase
MBS7217561 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Baculoviral IAP repeat containing protein 2 (BIRC2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BIRC2. The ELISA analytical biochemical technique of the MBS7217561 kit is based on BIRC2 antibody-BIRC2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BIRC2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BIRC2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BIRC2 elisa kit
Intended Uses: This BIRC2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BIRC2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Typical Testing Data/Standard Curve (for reference only) of BIRC2 elisa kit
BIRC2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7217561. Click to request current manual
NCBI/Uniprot data below describe general gene information for BIRC2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
64,098 Da
NCBI Official Full Name
baculoviral IAP repeat-containing protein 2 isoform 1
NCBI Official Synonym Full Names
baculoviral IAP repeat containing 2
NCBI Official Symbol
BIRC2  [Similar Products]
NCBI Official Synonym Symbols
API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
  [Similar Products]
NCBI Protein Information
baculoviral IAP repeat-containing protein 2; IAP-2; IAP homolog B; apoptosis inhibitor 1; RING finger protein 48; inhibitor of apoptosis protein 2; baculoviral IAP repeat-containing 2; NFR2-TRAF signalling complex protein; TNFR2-TRAF-signaling complex protein 2
UniProt Protein Name
Baculoviral IAP repeat-containing protein 2
UniProt Synonym Protein Names
C-IAP1; IAP homolog B; Inhibitor of apoptosis protein 2; IAP-2; hIAP-2; hIAP2; RING finger protein 48; TNFR2-TRAF-signaling complex protein 2
UniProt Gene Name
BIRC2  [Similar Products]
UniProt Synonym Gene Names
API1; IAP2; MIHB; RNF48; IAP-2; hIAP-2; hIAP2  [Similar Products]
UniProt Entry Name
BIRC2_HUMAN
NCBI Summary for BIRC2
The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Comments for BIRC2
BIRC2: Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin- protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO and MXD1/MAD1. Can also function as an E3 ubiquitin- protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle. Interacts with DIABLO/SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. Interacts with CASP9. Interacts (via BIR domains) with TRAF2. Interacts with E2F1, RIPK1, RIPK2, RIPK3, RIPK4, BIRC5/survivin and USP19. Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes. The CARD domain inhibits the activation of E3 ubiquitin ligase activity by preventing RING domain dimerization and E2 ubiquitin donor binding and activation. The CARD domain- mediated autoinhibition of the E3 ubiquitin-protein ligase activity suppresses cell proliferation and migration. USP19 regulates the stability of BIRC2/c-IAP1 by preventing its ubiquitination. Belongs to the IAP family.

Protein type: Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q22

Cellular Component: XY body; internal side of plasma membrane; spindle microtubule; cytoplasm; cytosol; nucleus; lipid raft

Molecular Function: protein binding; zinc ion binding; caspase inhibitor activity; transcription coactivator activity; ubiquitin-protein ligase activity; protein N-terminus binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; regulation of toll-like receptor signaling pathway; response to cAMP; protein polyubiquitination; regulation of cell cycle; apoptosis; protein heterooligomerization; toll-like receptor 3 signaling pathway; regulation of apoptosis; cell surface receptor linked signal transduction; regulation of transcription, DNA-dependent; regulation of cell differentiation; toll-like receptor 4 signaling pathway; placenta development; cell structure disassembly during apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; regulation of innate immune response; regulation of cell proliferation; response to ethanol; regulation of inflammatory response; toll-like receptor signaling pathway; response to hypoxia; innate immune response; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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