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BIRC7 elisa kit :: Porcine Anti-livin antibody ELISA Kit

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Catalog # MBS741171
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Anti-livin antibody (BIRC7), ELISA Kit

Full Product Name

Porcine Anti-livin antibody ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
605737
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This Anti-livin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine Anti-livin. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BIRC7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for BIRC7purchase
MBS741171 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-livin antibody (BIRC7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BIRC7. The ELISA analytical biochemical technique of the MBS741171 kit is based on BIRC7 antibody-BIRC7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BIRC7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BIRC7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BIRC7 elisa kit
Principle of the Assay: Anti-livin ELISA kit applies the competitive enzyme immunoassay technique utilizing Anti-livin antigen (Anti-livin) and an Anti-livin-HRP conjugate. The assay sample and buffer are incubated together with Anti-livin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-livin concentration since Anti-livin from samples and Anti-livin-HRP conjugate compete for the Anti-livin antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-livin from the sample, fewer sites are left to bind Anti-livin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-livin concentration in each sample is interpolated from this standard curve.
Product Categories/Family for BIRC7 elisa kit

Typical Testing Data/Standard Curve (for reference only) of BIRC7 elisa kit
BIRC7 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS741171. Click to request current manual
NCBI/Uniprot data below describe general gene information for BIRC7. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30,866 Da[Similar Products]
NCBI Official Full Name
LIVIN
NCBI Official Synonym Full Names
baculoviral IAP repeat containing 7
NCBI Official Symbol
BIRC7  [Similar Products]
NCBI Official Synonym Symbols
KIAP; LIVIN; MLIAP; RNF50; ML-IAP
  [Similar Products]
NCBI Protein Information
baculoviral IAP repeat-containing protein 7; RING finger protein 50; livin inhibitor of apoptosis; kidney inhibitor of apoptosis protein; melanoma inhibitor of apoptosis protein
UniProt Protein Name
Baculoviral IAP repeat-containing protein 7
UniProt Synonym Protein Names
Kidney inhibitor of apoptosis protein; KIAP; Livin; Melanoma inhibitor of apoptosis protein; ML-IAP; RING finger protein 50Cleaved into the following chain:Baculoviral IAP repeat-containing protein 7 30kDa subunit; Truncated livin; p30-Livin; tLivin
UniProt Gene Name
BIRC7  [Similar Products]
UniProt Synonym Gene Names
KIAP; LIVIN; MLIAP; RNF50; UNQ5800/PRO19607/PRO21344; KIAP; ML-IAP; Truncated livin; p30-Livin; tLivin  [Similar Products]
UniProt Entry Name
BIRC7_HUMAN
NCBI Summary for BIRC7
This gene encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jul 2013]
UniProt Comments for BIRC7
BIRC7: Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing. Binds to CASP9. Interaction with DIABLO/SMAC via the BIR domain disrupts binding to CASP9 and apoptotic suppressor activity. Interacts with TAB1. In vitro, interacts with CASP3 and CASP7 via its BIR domain. Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes. Belongs to the IAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ligase; Apoptosis; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: Golgi apparatus; spindle microtubule; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; cysteine protease inhibitor activity; ligase activity

Biological Process: protein ubiquitination; regulation of signal transduction; activation of JNK activity; regulation of cell proliferation; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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