• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

bla recombinant protein :: Beta-lactamase TEM Recombinant Protein

Scan QR to view Datasheet
Catalog # MBS1262737
Unit / Price
  0.01 mg (Yeast)  /  $110 +1 FREE 8GB USB
  0.01 mg (E-Coli)  /  $110 +1 FREE 8GB USB
  0.05 mg (Yeast)  /  $190 +1 FREE 8GB USB
  0.05 mg (E-Coli)  /  $190 +1 FREE 8GB USB
  0.1 mg (E-Coli)  /  $285 +1 FREE 8GB USB
  0.1 mg (Yeast)  /  $285 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $460 +1 FREE 8GB USB
  0.2 mg (Yeast)  /  $460 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $750 +1 FREE 8GB USB
  0.5 mg (Yeast)  /  $750 +1 FREE 8GB USB
  0.05 mg (Baculovirus)  /  $950 +1 FREE 8GB USB
  1 mg (E-Coli)  /  $1,180 +1 FREE 8GB USB
  1 mg (Yeast)  /  $1,180 +1 FREE 8GB USB
  0.05 mg (Mammalian-Cell)  /  $1,185 +1 FREE 8GB USB
  0.1 mg (Baculovirus)  /  $1,195 +1 FREE 8GB USB
  0.5 mg (Baculovirus)  /  $1,725 +2 FREE 8GB USB
  0.1 mg (Mammalian-Cell)  /  $1,805 +2 FREE 8GB USB
  1 mg (Baculovirus)  /  $2,295 +2 FREE 8GB USB
SDS-Page
Product Name

Beta-lactamase TEM (bla), Recombinant Protein

Popular Item
Also Known As

Recombinant Escherichia coli Beta-lactamase TEM

Product Synonym Names
IRT-4; Penicillinase; TEM-1; TEM-16/CAZ-7; TEM-2; TEM-24/CAZ-6; TEM-3; TEM-4; TEM-5; TEM-6; TEM-8/CAZ-2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS1262737 COA
Sequence Positions
24-286aa; Full Length
Sequence Length
286
Sequence
HPETLVKVKD AEDQLGARVG YIELDLNSGK ILESFRPEER FPMMSTFKVL LCGAVLSRVD AGQEQLGRRI HYSQNDLVEY SPVTEKHLTD GMTVRELCSA AITMSDNTAA NLLLTTIGGP KELTAFLHNM GDHVTRLDRW EPELNEAIPN DERDTTMPAA MATTLRKLLT GELLTLASRQ QLIDWMEADK VAGPLLRSAL PAGWFIADKS GAGERGSRGI IAALGPDGKP SRIVVIYTTG SQATMDERNR QIAEIGASLI KHW
3D Structure
ModBase 3D Structure for P62593
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Production Note
Special Offer: The E. coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E. coli host-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E. coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E. coli host-expressed protein for the fastest delivery among all hosts. Please contact us or email to support@mybiosource.com for more details.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of bla recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
bla recombinant protein
T-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. T-3 and T-4 are capable of hydrolyzing cefotaxime and ceftazidime. T-5 is capable of hydrolyzing ceftazidime. T-6 is capable of hydrolyzing ceftazidime and aztreonam. T-8/CAZ-2, T-16/CAZ-7 and T-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.

bla recombinant protein SDS-Page image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for bla. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44.89kD
NCBI Official Full Name
beta-lactamase (plasmid)
NCBI Official Symbol
pIGAL1_03  [Similar Products]
NCBI Protein Information
beta-lactamase
UniProt Protein Name
Beta-lactamase TEM
UniProt Synonym Protein Names
IRT-4; Penicillinase; TEM-1; TEM-16/CAZ-7; TEM-2; TEM-24/CAZ-6; TEM-3; TEM-4; TEM-5; TEM-6; TEM-8/CAZ-2
Protein Family
UniProt Gene Name
UniProt Entry Name
BLAT_ECOLX
UniProt Comments for bla
TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.
Product References and Citations for bla recombinant protein
Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.Sutcliffe J.G.Proc. Natl. Acad. Sci. U.S.A. 75:3737-3741(1978) Complete nucleotide sequence of the Escherichia coli plasmid pBR322.Sutcliffe J.G.Cold Spring Harb. Symp. Quant. Biol. 43:77-90(1979) DNA replication of the resistance plasmid R100 and its control.Ohtsubo H., Ryder T.B., Maeda Y., Armstrong K., Ohtsubo E.Adv. Biophys. 21:115-133(1986) Partial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K.Ambler R.P., Scott G.K.Proc. Natl. Acad. Sci. U.S.A. 75:3732-3736(1978) The TEM-3 beta-lactamase, which hydrolyzes broad-spectrum cephalosporins, is derived from the TEM-2 penicillinase by two amino acid substitutions.Sougakoff W., Goussard S., Courvalin P.FEMS Microbiol. Lett. 56:343-348(1988) A new example of physical linkage between Tn1 and Tn21 the antibiotic multiple-resistance region of plasmid pCFF04 encoding extended-spectrum beta-lactamase TEM-3.Mabilat C., Lourencao-Vital J., Goussard S., Courvalin P.Mol. Gen. Genet. 235:113-121(1992) Characterization of the plasmid genes blaT-4 and blaT-5 which encode the broad-spectrum beta-lactamases TEM-4 and TEM-5 in enterobacteriaceae.Sougakoff W., Petit A., Goussard S., Sirot D., Bure A., Courvalin P.Gene 78:339-348(1989) An IS1-like element is responsible for high-level synthesis of extended-spectrum beta-lactamase TEM-6 in Enterobacteriaceae.Goussard S., Sougakoff W., Mabilat C., Bauernfeind A., Courvalin P.J. Gen. Microbiol. 137:2681-2687(1991) Nucleotide sequences of CAZ-2, CAZ-6, and CAZ-7 beta-lactamase genes.Chanal C., Poupart M.C., Sirot D., Labia R., Sirot J., Cluzel R.Antimicrob. Agents Chemother. 36:1817-1820(1992) Characterization and amino acid sequence of IRT-4, a novel TEM-type enzyme with a decreased susceptibility to beta-lactamase inhibitors.Brun T., Peduzzi J., Canica M.M., Paul G., Nevot P., Barthelemy M., Labia R.FEMS Microbiol. Lett. 120:111-117(1994) Beta-lactamase TEM1 of E. coli. Crystal structure determination at 2.5-A resolution.Jelsch C., Lenfant F., Masson J.-M., Samama J.-P.FEBS Lett. 299:135-142(1992) Crystal structure of Escherichia coli TEM1 beta-lactamase at 1.8-A resolution.Jelsch C., Mourey L., Masson J.-M., Samama J.-P.Proteins 16:364-383(1993) A potent new mode of beta-lactamase inhibition revealed by the 1.7 A X-ray crystallographic structure of the TEM-1-BLIP complex.Strynadka N.C.J., Jensen S.E., Alzari P.M., James M.N.G.Nat. Struct. Biol. 3:290-297(1996) Crystal structure of an acylation transition-state analog of the TEM-1 beta-lactamase. Mechanistic implications for class A beta-lactamases.Maveyraud L., Pratt R.F., Samama J.-P.Biochemistry 37:2622-2628(1998) X-ray structure of the Asn276Asp variant of the Escherichia coli TEM-1 beta-lactamase direct observation of electrostatic modulation in resistance to inactivation by clavulanic acid.Swaren P., Golemi D., Cabantous S., Bulychev A., Maveyraud L., Mobashery S., Samama J.-P.Biochemistry 38:9570-9576(1999)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000