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BMP-1 elisa kit :: Sheep BMP-1 ELISA Kit

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Catalog # MBS2501867
Unit / Price
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  48-Strip-Wells  /  $490 +1 FREE 8GB USB
  96-Strip-Wells  /  $585 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,475 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,360 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

BMP-1, ELISA Kit

Full Product Name

Sheep BMP-1 (Bone Morphogenetic Protein 1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
112264
3D Structure
ModBase 3D Structure for P13497
Species Reactivity
Specificity
This kit recognizes natural and recombinant Sheep BMP-1. No significant cross-reactivity or interference between Sheep BMP-1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.781-50ng/mL
Sensitivity
Min: 0.469ng/mL; Max: 50ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BMP-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for BMP-1purchase
MBS2501867 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BMP-1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BMP-1. The ELISA analytical biochemical technique of the MBS2501867 kit is based on BMP-1 antibody-BMP-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BMP-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BMP-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BMP-1 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep BMP-1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BMP-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BMP-1. You can calculate the concentration of BMP-1 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of BMP-1 elisa kit
BMP-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2501867. Click to request current manual
NCBI/Uniprot data below describe general gene information for BMP-1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
92,655 Da
NCBI Official Full Name
bone morphogenetic protein, partial
NCBI Official Synonym Full Names
bone morphogenetic protein 1
NCBI Official Symbol
NCBI Official Synonym Symbols
PCP; TLD; OI13; PCP2; PCOLC
  [Similar Products]
NCBI Protein Information
bone morphogenetic protein 1; mammalian tolloid protein; procollagen C-endopeptidase; procollagen C-proteinase 3
UniProt Protein Name
Bone morphogenetic protein 1
UniProt Synonym Protein Names
Mammalian tolloid protein; mTld; Procollagen C-proteinase; PCP
UniProt Gene Name
UniProt Synonym Gene Names
PCOLC; BMP-1; mTld; PCP  [Similar Products]
UniProt Entry Name
BMP1_HUMAN
NCBI Summary for BMP-1
This gene encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, this gene encodes a protein that is not closely related to other known growth factors. This gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region. [provided by RefSeq, Aug 2008]
UniProt Comments for BMP-1
BMP1: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Defects in BMP1 are a cause of autosomal recessive osteogenesis imperfecta (AR-OI). A connective tissue disorder characterized by bone fragility, progressively deforming bones, bowing of limbs due to multiple fractures, very short stature, a triangular face, severe scoliosis, and grayish sclera. AR-OI due to BMP1 mutations belongs to the group of osteogenesis imperfecta type III in the Sillence classification. Belongs to the peptidase M12A family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.19; Protease; Cytokine

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: peptidase activity; growth factor activity; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; cytokine activity; calcium ion binding

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; ossification; multicellular organismal development; lipoprotein metabolic process; proteolysis; cell differentiation; skeletal development; cartilage condensation

Disease: Osteogenesis Imperfecta, Type Xiii
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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