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BMP-2 elisa kit :: Rat BMP-2 ELISA Kit

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Catalog # MBS355415
Unit / Price
  96 Tests  /  $455 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

BMP-2, ELISA Kit

Popular Item
Full Product Name

BMP-2 Rat ELISA Kit

Product Synonym Names
Rat BMP-2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 20; NC_000020.10 (6748745..6760910). Location: 20p12
OMIM
112261
3D Structure
ModBase 3D Structure for P12643
Species Reactivity
Assay Type
Sandwich
Detection Range
62.5 pg/ml-4000 pg/ml
Sensitivity
< 2pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BMP-2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forBMP-2purchase
MBS355415 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BMP-2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BMP-2. The ELISA analytical biochemical technique of the MBS355415 kit is based on BMP-2 antibody-BMP-2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BMP-2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BMP-2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BMP-2 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BMP-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BMP-2 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BMP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BMP-2 can be calculated.

Background: Bone morphogenetic protein 2 (BMP-2) belongs to the TGF-beta superfamily of proteins. Like other family members, it plays an important role in the development of bone and cartilage. It has been demonstrated to potently induce osteoblast differentiation in a variety of cell types.BMP-2 has an ability to induce the formation of ectopic cartilage when implanted subcutaneously.
Applications Tested/Suitable for BMP-2 elisa kit
Quantitative sELISA (EIA)
Application Notes for BMP-2 elisa kit
For quantitative detection of BMP-2 in serum, plasma, body fluids, tissue lysates or cell culture supernates.

Typical Testing Data/Standard Curve (for reference only) of BMP-2 elisa kit
BMP-2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS355415. Click to request current manual
NCBI/Uniprot data below describe general gene information for BMP-2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
44,702 Da
NCBI Official Full Name
bone morphogenetic protein 2
NCBI Official Synonym Full Names
bone morphogenetic protein 2
NCBI Official Symbol
NCBI Official Synonym Symbols
BDA2; BMP2A
  [Similar Products]
NCBI Protein Information
bone morphogenetic protein 2; BMP-2A; bone morphogenetic protein 2A
UniProt Protein Name
Bone morphogenetic protein 2
UniProt Synonym Protein Names
Bone morphogenetic protein 2A
UniProt Gene Name
UniProt Synonym Gene Names
BMP2A; BMP-2; BMP-2A  [Similar Products]
UniProt Entry Name
BMP2_HUMAN
NCBI Summary for BMP-2
The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]
UniProt Comments for BMP-2
Function: Induces cartilage and bone formation.

Subunit structure: Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with GREM2, RGMA, RGMB and RGMC. Interacts with ASPN

By similarity. Ref.4

Subcellular location: Secreted.

Tissue specificity: Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

Pharmaceutical use: Available under the name Infuse (Medtronic Sofamor Danek). Used for treating open tibial shaft fractures.

Sequence similarities: Belongs to the TGF-beta family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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