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BMPR1A elisa kit :: Rat Bone Morphogenetic Protein Receptor 1A ELISA Kit

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Catalog # MBS720872
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Bone Morphogenetic Protein Receptor 1A (BMPR1A), ELISA Kit

Popular Item
Full Product Name

Rat Bone Morphogenetic Protein Receptor 1A ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
612242
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of BMPR1A. No significant cross-reactivity or interference between BMPR1A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BMPR1A and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1 ng/ml
Intended Uses
This BMPR1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat BMPR1A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BMPR1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for BMPR1Apurchase
MBS720872 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BMPR1A. The ELISA analytical biochemical technique of the MBS720872 kit is based on BMPR1A antibody-BMPR1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BMPR1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BMPR1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BMPR1A elisa kit
Principle of the assay: BMPR1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMPR1A antibody and an BMPR1A-HRP conjugate. The assay sample and buffer are incubated together with BMPR1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMPR1A concentration since BMPR1A from samples and BMPR1A-HRP conjugate compete for the anti-BMPR1A antibody binding site. Since the number of sites is limited, as more sites are occupied by BMPR1A from the sample, fewer sites are left to bind BMPR1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMPR1A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for BMPR1A elisa kit

Typical Testing Data/Standard Curve (for reference only) of BMPR1A elisa kit
BMPR1A elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS720872. Click to request current manual
NCBI/Uniprot data below describe general gene information for BMPR1A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
60,198 Da
NCBI Official Full Name
bone morphogenetic protein receptor type-1A
NCBI Official Synonym Full Names
bone morphogenetic protein receptor, type IA
NCBI Official Symbol
BMPR1A  [Similar Products]
NCBI Official Synonym Symbols
ALK3; SKR5; CD292; ACVRLK3; 10q23del
  [Similar Products]
NCBI Protein Information
bone morphogenetic protein receptor type-1A; ALK-3; BMPR-1A; BMP type-1A receptor; activin receptor-like kinase 3; activin A receptor, type II-like kinase 3; serine/threonine-protein kinase receptor R5
UniProt Protein Name
Bone morphogenetic protein receptor type-1A
UniProt Synonym Protein Names
Activin receptor-like kinase 3; ALK-3; Serine/threonine-protein kinase receptor R5; SKR5; CD_antigen: CD292
UniProt Gene Name
BMPR1A  [Similar Products]
UniProt Synonym Gene Names
ACVRLK3; ALK3; BMP type-1A receptor; BMPR-1A; ALK-3; SKR5  [Similar Products]
UniProt Entry Name
BMR1A_HUMAN
NCBI Summary for BMPR1A
The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. [provided by RefSeq, Jul 2008]
UniProt Comments for BMPR1A
BMPR1A: a serine/threonine-protein kinase receptor for Bone morphogenetic protein-2 and -4 (BMP-2 and BMP-4). Defects in BMPR1A are a cause of juvenile polyposis syndrome (JPS) and Cowden disease (CD), a cancer syndrome characterized by multiple hamartomas and by a high risk for breast, thyroid and endometriel cancers.

Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); EC 2.7.11.30; TKL group; STKR family; Type1 subfamily

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: cell soma; dendrite; plasma membrane; integral to membrane; caveola

Molecular Function: transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; metal ion binding; SMAD binding; transmembrane receptor protein serine/threonine kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: neural plate mediolateral pattern formation; transcription from RNA polymerase II promoter; hindlimb morphogenesis; developmental growth; neural crest cell development; positive regulation of transcription, DNA-dependent; mesendoderm development; paraxial mesoderm structural organization; dorsal/ventral axis specification; palate development; protein amino acid phosphorylation; negative regulation of neurogenesis; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; positive regulation of mesenchymal cell proliferation; ectoderm development; Mullerian duct regression; somitogenesis; in utero embryonic development; stem cell maintenance; lateral mesoderm development; positive regulation of bone mineralization; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; mesoderm formation; pituitary gland development; cartilage development; embryonic organ development; immune response; embryonic digit morphogenesis; positive regulation of epithelial cell proliferation; regulation of lateral mesodermal cell fate specification; lung development

Disease: Juvenile Polyposis Syndrome; Polyposis Syndrome, Hereditary Mixed, 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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