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BMPR1B elisa kit :: Rat Bone Morphogenetic Protein Receptor 1B ELISA Kit

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Catalog # MBS753333
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Bone Morphogenetic Protein Receptor 1B (BMPR1B), ELISA Kit

Popular Item
Full Product Name

Rat Bone Morphogenetic Protein Receptor 1B ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 609441
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of BMPR1B. No significant cross-reactivity or interference between BMPR1B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BMPR1B and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1 ng/ml
Intended Uses
This BMPR1B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat BMPR1B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BMPR1B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forBMPR1Bpurchase
MBS753333 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bone Morphogenetic Protein Receptor 1B (BMPR1B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BMPR1B. The ELISA analytical biochemical technique of the MBS753333 kit is based on BMPR1B antibody-BMPR1B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BMPR1B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BMPR1B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BMPR1B elisa kit

Principle of the assay: BMPR1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMPR1B antibody and an BMPR1B-HRP conjugate. The assay sample and buffer are incubated together with BMPR1B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMPR1B concentration since BMPR1B from samples and BMPR1B-HRP conjugate compete for the anti-BMPR1B antibody binding site. Since the number of sites is limited, as more sites are occupied by BMPR1B from the sample, fewer sites are left to bind BMPR1B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMPR1B concentration in each sample is interpolated from this standard curve.
Product Categories/Family for BMPR1B elisa kit

Typical Testing Data/Standard Curve (for reference only) of BMPR1B elisa kit
BMPR1B elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS753333. Click to request current manual
NCBI/Uniprot data below describe general gene information for BMPR1B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
60,582 Da
NCBI Official Full Name
bone morphogenetic protein receptor type-1B isoform b
NCBI Official Synonym Full Names
bone morphogenetic protein receptor, type IB
NCBI Official Symbol
BMPR1B  [Similar Products]
NCBI Official Synonym Symbols
ALK6; ALK-6; CDw293
  [Similar Products]
NCBI Protein Information
bone morphogenetic protein receptor type-1B; BMPR-1B; BMP type-1B receptor; activin receptor-like kinase 6; serine/threonine receptor kinase
UniProt Protein Name
Bone morphogenetic protein receptor type-1B
UniProt Synonym Protein Names
CD_antigen: CDw293
UniProt Gene Name
BMPR1B  [Similar Products]
UniProt Synonym Gene Names
BMP type-1B receptor; BMPR-1B  [Similar Products]
UniProt Entry Name
BMR1B_HUMAN
NCBI Summary for BMPR1B
This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
UniProt Comments for BMPR1B
BMPR1B: a kinase of the STKR family. A type I cell-surface receptor for bone morphogenetic protein. Expressed at ighest level in the prostate. May be upregulated by androgen stimulation in cells which expresses the androgen receptor.

Protein type: Kinase, protein; EC 2.7.11.30; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; Membrane protein, integral; TKL group; STKR family; Type1 subfamily

Chromosomal Location of Human Ortholog: 4q22-q24

Cellular Component: integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: protein serine/threonine kinase activity; protein binding; transforming growth factor beta receptor activity, type I; metal ion binding; SMAD binding; ATP binding; transmembrane receptor protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: chondrocyte development; positive regulation of bone mineralization; limb morphogenesis; protein amino acid phosphorylation; BMP signaling pathway; positive regulation of osteoblast differentiation; ovulation cycle; eye development; dorsal/ventral pattern formation; retina development in camera-type eye; proteoglycan biosynthetic process; ovarian cumulus expansion; positive regulation of cell differentiation; inflammatory response; skeletal development; retinal ganglion cell axon guidance; cartilage condensation

Disease: Chondrodysplasia, Acromesomelic, With Genital Anomalies; Brachydactyly, Type A2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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