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BOLA3 elisa kit :: Mouse BolA-like protein 3 (BOLA3) ELISA Kit

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Catalog # MBS7207186
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

BolA-like protein 3 (BOLA3), ELISA Kit

Popular Item
Also Known As

Mouse BolA-like protein 3 (BOLA3) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 614299
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of BOLA3. No significant cross-reactivity or interference between BOLA3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BOLA3 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Intended Uses
This BOLA3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BOLA3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BOLA3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forBOLA3purchase
MBS7207186 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BolA-like protein 3 (BOLA3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BOLA3. The ELISA analytical biochemical technique of the MBS7207186 kit is based on BOLA3 antibody-BOLA3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BOLA3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BOLA3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BOLA3 elisa kit
Principle of the assay: BOLA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BOLA3 antibody and an BOLA3-HRP conjugate. The assay sample and buffer are incubated together with BOLA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BOLA3 concentration since BOLA3 from samples and BOLA3-HRP conjugate compete for the anti-BOLA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by BOLA3 from the sample, fewer sites are left to bind BOLA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BOLA3 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for BOLA3 elisa kit

Typical Testing Data/Standard Curve (for reference only) of BOLA3 elisa kit
BOLA3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7207186. Click to request current manual
NCBI/Uniprot data below describe general gene information for BOLA3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,569 Da
NCBI Official Full Name
bolA-like protein 3 isoform 1
NCBI Official Synonym Full Names
bolA family member 3
NCBI Official Symbol
BOLA3  [Similar Products]
NCBI Official Synonym Symbols
MMDS2
  [Similar Products]
NCBI Protein Information
bolA-like protein 3; bolA homolog 3
UniProt Protein Name
BolA-like protein 3
Protein Family
UniProt Gene Name
BOLA3  [Similar Products]
UniProt Entry Name
BOLA3_HUMAN
NCBI Summary for BOLA3
This gene encodes a protein that plays an essential role in the production of iron-sulfur (Fe-S) clusters for the normal maturation of lipoate-containing 2-oxoacid dehydrogenases, and for the assembly of the mitochondrial respiratory chain complexes. Mutation in this gene has been associated with multiple mitochondrial dysfunctions syndrome-2. Two alternatively spliced transcript variants encoding different isoforms with distinct subcellular localization have been reported for this gene (PMID:21944046). [provided by RefSeq, Dec 2011]
UniProt Comments for BOLA3
BOLA3: Defects in BOLA3 are the cause of multiple mitochondrial dysfunctions syndrome type 2 (MMDS2). A severe disorder of systemic energy metabolism, resulting in weakness, respiratory failure, lack of neurologic development, lactic acidosis, hyperglycinemia and early death. Some patients show failure to thrive, pulmonary hypertension, hypotonia and irritability. Biochemical features include severe combined deficiency of the 2-oxoacid dehydrogenases, defective lipoic acid synthesis and reduction in activity of mitochondrial respiratory chain complexes. Belongs to the bolA/yrbA family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: extracellular region

Disease: Multiple Mitochondrial Dysfunctions Syndrome 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Diseases associated with BOLA3 elisa kit
 Disease Name  Pubmed Publications
 Atrophy Antibodies  >1 publications with BOLA3 and Atrophy
Organs/Tissues associated with BOLA3 elisa kit
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