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botA recombinant protein :: Botulinum neurotoxin type A Recombinant Protein

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Catalog # MBS1068389
Unit / Price
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  0.01 mg (E-Coli)  /  $235 +1 FREE 8GB USB
  0.01 mg (Yeast)  /  $260 +1 FREE 8GB USB
  0.05 mg (E-Coli)  /  $320 +1 FREE 8GB USB
  0.05 mg (Yeast)  /  $360 +1 FREE 8GB USB
  0.1 mg (E-Coli)  /  $535 +1 FREE 8GB USB
  0.1 mg (Yeast)  /  $595 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $855 +1 FREE 8GB USB
  0.2 mg (Yeast)  /  $965 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $1,125 +1 FREE 8GB USB
  0.5 mg (Yeast)  /  $1,345 +1 FREE 8GB USB
  1 mg (E-Coli)  /  $1,725 +2 FREE 8GB USB
  1 mg (Yeast)  /  $2,065 +2 FREE 8GB USB
SDS-PAGE
Product Name

Botulinum neurotoxin type A (botA), Recombinant Protein

Full Product Name

Recombinant Clostridium botulinum Botulinum neurotoxin type A

Product Synonym Names
Bontoxilysin-A; BOTOX
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-436aa; Partial
Sequence
MPFVNKQFNY KDPVNGVDIA YIKIPNVGQM QPVKAFKIHN KIWVIPERDT FTNPEEGDLN PPPEAKQVPV SYYDSTYLST DNEKDNYLKG VTKLFERIYS TDLGRMLLTS IVRGIPFWGG STIDTELKVI DTNCINVIQP DGSYRSEELN LVIIGPSADI IQFECKSFGH EVLNLTRNGY GSTQYIRFSP DFTFGFEESL EVDTNPLLGA GKFATDPAVT LAHELIHAGH RLYGIAINPN RVFKVNTNAY YEMSGLEVSF EELRTFGGHD AKFIDSLQEN EFRLYYYNKF KDIASTLNKA KSIVGTTASL QYMKNVFKEK YLLSEDTSGK FSVDKLKFDK LYKMLTEIYT EDNFVKFFKV LNRKTYLNFD KAVFKINIVP KVNYTIYDGF NLRNTNLAAN FNGQNTEINN MNFTKLKNFT GLFEFYKLLC VRGIIT
3D Structure
ModBase 3D Structure for P10845
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of botA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
botA recombinant protein
Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.

botA recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for botA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
65.94kD
NCBI Official Full Name
peptidase M27
UniProt Protein Name
Botulinum neurotoxin type A
UniProt Synonym Protein Names
Bontoxilysin-A; BOTOX
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
atx; bna; BoNT/A; BOTOX  [Similar Products]
UniProt Entry Name
BXA1_CLOBO
UniProt Comments for botA
Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
Product References and Citations for botA recombinant protein
The complete amino acid sequence of the Clostridium botulinum type A neurotoxin, deduced by nucleotide sequence analysis of the encoding gene.Thompson D.E., Brehm J.K., Oultram J.D., Swinfield T.-J., Shone C.C., Atkinson T., Melling J., Minton N.P.Eur. J. Biochem. 189:73-81(1990) The complete sequence of botulinum neurotoxin type A and comparison with other clostridial neurotoxins.Binz T., Kurazono H., Wille M., Frevert J., Wernars K., Niemann H.J. Biol. Chem. 265:9153-9158(1990) Organization and phylogenetic interrelationships of genes encoding components of the botulinum toxin complex in proteolytic Clostridium botulinum types A, B, and F evidence of chimeric sequences in the gene encoding the nontoxic nonhemagglutinin component.East A.K., Bhandari M., Stacey J.M., Campbell K.D., Collins M.D.Int. J. Syst. Bacteriol. 46:1105-1112(1996) Molecular characterization of two forms of nontoxic-nonhemagglutinin components of Clostridium botulinum type A progenitor toxins.Fujita R., Fujinaga Y., Inoue K., Nakajima H., Kumon H., Oguma K.FEBS Lett. 376:41-44(1995) Partial amino acid sequence of the heavy and light chains of botulinum neurotoxin type A.Schmidt J.J., Sartymoorthy V., Dasgupta B.R.Biochem. Biophys. Res. Commun. 119:900-904(1984) Partial sequence of the light chain of botulinum neurotoxin type A.Dasgupta B.R., Foley J., Niece R.Biochemistry 26:4162-4162(1987) Botulinum neurotoxin type A sequence of amino acids at the N-terminus and around the nicking site.Dasgupta B.R., Dekleva M.L.Biochimie 72:661-664(1990) Botulinum neurotoxin type A cleavage of the heavy chain into two halves and their partial sequences.Sathymoorthy V., Dasgupta B.R., Foley J., Niece R.L.Arch. Biochem. Biophys. 266:142-151(1988) Inactivation of Clostridium botulinum type A neurotoxin by trypsin and purification of two tryptic fragments. Proteolytic action near the COOH-terminus of the heavy subunit destroys toxin-binding activity.Shone C.C., Hambleton P., Melling J.Eur. J. Biochem. 151:75-82(1985) Botulinum neurotoxins serotypes A and E cleave SNAP-25 at distinct COOH-terminal peptide bonds.Schiavo G., Santtuci A., Dasgupta B.R., Mehta P.P., Jontes J., Benfenati F., Wilson M.C., Montecucco C.FEBS Lett. 335:99-103(1993) Site-directed mutagenesis identifies active-site residues of the light chain of botulinum neurotoxin type a.Rigoni M., Caccin P., Johnson E.A., Montecucco C., Rossetto O.Biochem. Biophys. Res. Commun. 288:1231-1237(2001) SV2 is the protein receptor for botulinum neurotoxin A.Dong M., Yeh F., Tepp W.H., Dean C., Johnson E.A., Janz R., Chapman E.R.Science 312:592-596(2006) Crystal structure of botulinum neurotoxin type A and implications for toxicity.Lacy D.B., Tepp W., Cohen A.C., Dasgupta B.R., Stevens R.C.Nat. Struct. Biol. 5:898-902(1998)

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Disclaimer
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Products associated with botA recombinant protein
 Reference Product  PubMed Publications
 ntnH recombinant protein  >2 publications with botA and ntnH
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