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BP180-Ab elisa kit :: Rat Anti-bullous pemphigoid 180 antibody ELISA Kit

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Catalog # MBS731382
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Anti-bullous pemphigoid 180 antibody (BP180-Ab), ELISA Kit

Popular Item
Also Known As

Rat Anti-bullous pemphigoid 180 antibody ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 226650
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of BP180-Ab. No significant cross-reactivity or interference between BP180-Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BP180-Ab and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BP180-Ab elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forBP180-Abpurchase
MBS731382 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-bullous pemphigoid 180 antibody (BP180-Ab) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BP180-Ab. The ELISA analytical biochemical technique of the MBS731382 kit is based on BP180-Ab antibody-BP180-Ab antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BP180-Ab antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BP180-Ab. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BP180-Ab elisa kit
Intended Uses: This BP180-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat BP180-Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: BP180-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Bullous Pemphigoid 180 antigen and an BP180-Ab-HRP conjugate. The assay sample and buffer are incubated together with BP180-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times.The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BP180-Ab concentration since BP180-Ab from samples and BP180-Ab-HRP conjugate compete for the Bullous Pemphigoid 180 antigen binding site. Since the number of sites is limited, as more sites are occupied by BP180-Ab from the sample, fewer sites are left to bind BP180-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BP180-Ab concentration in each sample is interpolated from this standard curve.
Product Categories/Family for BP180-Ab elisa kit

Typical Standard Curve/Testing Data of BP180-Ab elisa kit
BP180-Ab elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS731382. Click to request current manual
NCBI/Uniprot data below describe general gene information for BP180-Ab. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
142,344 Da
NCBI Official Full Name
180 kDa bullous pemphigoid antigen 2/type XVII collagen
NCBI Official Synonym Full Names
collagen, type XVII, alpha 1
NCBI Official Symbol
COL17A1  [Similar Products]
NCBI Official Synonym Symbols
BP180; BPA-2; BPAG2; LAD-1; BA16H23.2
  [Similar Products]
NCBI Protein Information
collagen alpha-1(XVII) chain; collagen alpha-1(XVII) chain; alpha 1 type XVII collagen; type XVII collagen alpha-1; collagen XVII, alpha-1 polypeptide; 180 kDa bullous pemphigoid antigen 2; bullous pemphigoid antigen 2 (180kD); bA16H23.2 (collagen, type XVII, alpha 1 (BP180))
UniProt Protein Name
Collagen alpha-1(XVII) chain
UniProt Synonym Protein Names
180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2Cleaved into the following 2 chains:120 kDa linear IgA disease antigenAlternative name(s):120 kDa linear IgA dermatosis antigen; Linear IgA disease antigen 1; LAD-1
UniProt Gene Name
COL17A1  [Similar Products]
UniProt Synonym Gene Names
BP180; BPAG2; LAD-1; 97 kDa LAD antigen; 97-LAD; LABD97  [Similar Products]
UniProt Entry Name
COHA1_HUMAN
NCBI Summary for BP180-Ab
This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]
UniProt Comments for BP180-Ab
COL17A1: May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane. Defects in COL17A1 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). GABEB is a non- lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Extracellular matrix; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q24.3

Cellular Component: collagen; hemidesmosome; integral to plasma membrane; endoplasmic reticulum lumen; extracellular region; plasma membrane; basement membrane; intercellular junction

Molecular Function: protein binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; epidermis development; extracellular matrix organization and biogenesis; hemidesmosome assembly; cell-matrix adhesion

Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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