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BPA elisa kit :: Mouse Bisphenol A (BPA) ELISA Kit

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Catalog # MBS2600653
Unit / Price
  48-Strip-Wells  /  $275 +1 FREE 8GB USB
  96-Strip-Wells  /  $435 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Bisphenol A (BPA), ELISA Kit

Popular Item
Also Known As

Mouse Bisphenol A (BPA) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
435
OMIM
602969
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant BPA concentration
Assay Type
Sandwich
Detection Range
200 ng/ml-3.12 ng/ml
Sensitivity
0.6 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BPA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forBPApurchase
MBS2600653 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bisphenol A (BPA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BPA. The ELISA analytical biochemical technique of the MBS2600653 kit is based on BPA antibody-BPA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BPA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BPA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BPA elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse BPA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of BPA elisa kit
BPA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2600653. Click to request current manual
NCBI/Uniprot data below describe general gene information for BPA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
52,462 Da
NCBI Official Full Name
bisphenol A receptor ERRgamma2
NCBI Official Synonym Full Names
estrogen-related receptor gamma
NCBI Official Symbol
ESRRG  [Similar Products]
NCBI Official Synonym Symbols
ERR3; NR3B3; ERRgamma
  [Similar Products]
NCBI Protein Information
estrogen-related receptor gamma; ERR gamma-2; estrogen receptor-related protein 3; nuclear receptor subfamily 3 group B member 3
UniProt Protein Name
Estrogen-related receptor gamma
UniProt Synonym Protein Names
ERR gamma-2; Estrogen receptor-related protein 3; Nuclear receptor subfamily 3 group B member 3
UniProt Gene Name
ESRRG  [Similar Products]
UniProt Synonym Gene Names
ERR3; ERRG2; KIAA0832; NR3B3  [Similar Products]
UniProt Entry Name
ERR3_HUMAN
NCBI Summary for BPA
This gene encodes a member of the estrogen receptor-related receptor (ESRR) family, which belongs to the nuclear hormone receptor superfamily. All members of the ESRR family share an almost identical DNA binding domain, which is composed of two C4-type zinc finger motifs. The ESRR members are orphan nuclear receptors; they bind to the estrogen response element and steroidogenic factor 1 response element, and activate genes controlled by both response elements in the absence of any ligands. The ESRR family is closely related to the estrogen receptor (ER) family. They share target genes, co-regulators and promoters, and by targeting the same set of genes, the ESRRs seem to interfere with the ER-mediated estrogen response in various ways. It has been reported that the family member encoded by this gene functions as a transcriptional activator of DNA cytosine-5-methyltransferases 1 (Dnmt1) expression by direct binding to its response elements in the DNMT1 promoters, modulates cell proliferation and estrogen signaling in breast cancer, and negatively regulates bone morphogenetic protein 2-induced osteoblast differentiation and bone formation. Multiple alternatively spliced transcript variants have been identified, which mainly differ at the 5' end and some of which encode protein isoforms differing in the N-terminal region. [provided by RefSeq, Aug 2011]
UniProt Comments for BPA
ERR3: Orphan receptor that acts as transcription activator in the absence of bound ligand. Binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements. Belongs to the nuclear hormone receptor family. NR3 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: nucleoplasm

Molecular Function: protein binding; zinc ion binding; AF-2 domain binding; steroid hormone receptor activity; retinoic acid receptor activity; steroid binding

Biological Process: retinoic acid receptor signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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