• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-BRAF antibody :: Rabbit BRAF-S445 Antibody

Scan QR to view Datasheet
Catalog # MBS125740
Unit / Price
  0.05 mL  /  $265 +1 FREE 8GB USB
  0.1 mL  /  $380 +1 FREE 8GB USB
  0.2 mL  /  $555 +1 FREE 8GB USB
Western Blot (WB)
Product Name

BRAF-S445, Antibody

Popular Item
Also Known As

Phospho-BRAF-S445

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
766
OMIM
phenotype 613707
3D Structure
ModBase 3D Structure for P15056
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity purification
Swiss Prot
P15056
Species
Human
Route
Synthetic Peptide
Immunogen
A phospho specific peptide corresponding to residues surrounding S445 of human BRAF.
Buffer
PBS with 0.02% sodium azide, 50% glyverol, pH7.3
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-BRAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-BRAF antibody
Applications Tested/Suitable for anti-BRAF antibody
Western Blot (WB)
Application Notes for anti-BRAF antibody
WB: 1:500 - 1:2000

Western Blot (WB) of anti-BRAF antibody
Western blot analysis of extracts of Raji cells, using Phospho-BRAF-S445 antibody at 1:2000 dilution. Raji cells were treated by PMA/TPA (200nM) for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s.
anti-BRAF antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for BRAF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
84kDa
NCBI Official Full Name
Serine/threonine-protein kinase B-raf
NCBI Official Synonym Full Names
B-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
NS7; BRAF1; RAFB1; B-RAF1
  [Similar Products]
NCBI Protein Information
serine/threonine-protein kinase B-raf; 94 kDa B-raf protein; proto-oncogene B-Raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94)
UniProt Protein Name
Serine/threonine-protein kinase B-raf
UniProt Synonym Protein Names
Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
UniProt Gene Name
UniProt Synonym Gene Names
BRAF1; RAFB1  [Similar Products]
UniProt Entry Name
BRAF_HUMAN
NCBI Summary for BRAF
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for BRAF
BRAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Oncoprotein; EC 2.7.11.1; TKL group; RAF family

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: neuron projection; mitochondrion; plasma membrane; cytosol; nucleus

Molecular Function: small GTPase binding; protein serine/threonine kinase activity; identical protein binding; protein binding; MAP kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; protein heterodimerization activity; calcium ion binding; ATP binding; protein kinase activity

Biological Process: positive T cell selection; response to peptide hormone stimulus; fibroblast growth factor receptor signaling pathway; response to cAMP; CD4-positive, alpha beta T cell differentiation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; somatic stem cell maintenance; protein heterooligomerization; MAPKKK cascade; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; regulation of cell proliferation; synaptic transmission; organ morphogenesis; myeloid progenitor cell differentiation; small GTPase mediated signal transduction; positive regulation of stress fiber formation; visual learning; negative regulation of neuron apoptosis; negative regulation of apoptosis

Disease: Noonan Syndrome 1; Noonan Syndrome 7; Lung Cancer; Cardiofaciocutaneous Syndrome 1; Leopard Syndrome 3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000