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BRD4 elisa kit :: Human bromodomain containing 4 ELISA Kit

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Catalog # MBS945281
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Typical Testing Data/Standard Curve (for reference only)
Product Name

bromodomain containing 4 (BRD4), ELISA Kit

Popular Item
Full Product Name

Human Bromodomain-containing protein 4, BRD4 ELISA Kit

Product Synonym Names
Human Bromodomain-containing protein 4 (BRD4) ELISA kit; CAP; HUNK1; HUNKI; MCAP; bromodomain-containing 4; bromodomain-containing protein 4; chromosome-associated protein; bromodomain containing 4
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS945281 Testing Data
MBS945281 MSDS
3D Structure
ModBase 3D Structure for O60885
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of human BRD4. No significant cross-reactivity or interference between human BRD4 and analogues was observed.
serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
18.75 pg/ml -1200 pg/ml
<4.68 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BRD4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for BRD4purchase
MBS945281 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the bromodomain containing 4 (BRD4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BRD4. The ELISA analytical biochemical technique of the MBS945281 kit is based on BRD4 antibody-BRD4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BRD4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BRD4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BRD4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BRD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BRD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BRD4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BRD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of BRD4 elisa kit
BRD4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS945281. Click to request current manual
NCBI/Uniprot data below describe general gene information for BRD4. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
152,219 Da
NCBI Official Full Name
bromodomain-containing protein 4 isoform short
NCBI Official Synonym Full Names
bromodomain containing 4
NCBI Official Symbol
NCBI Official Synonym Symbols
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NCBI Protein Information
bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein
UniProt Protein Name
Bromodomain-containing protein 4
UniProt Synonym Protein Names
Protein HUNK1
UniProt Gene Name
UniProt Synonym Gene Names
HUNK1  [Similar Products]
UniProt Entry Name
NCBI Summary for BRD4
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
UniProt Comments for BRD4
BRD4: an atypical protein kinase of the Brd family. Contains a double bromodomain. Binds to chromatin and regulates cell cycle progression at multiple stages. Associates with chromosomes during mitosis. The cognate gene is the chromosome 19 target of translocation t(15;19)(q13;p13.1), resulting in the BRD4-NUT oncogene identified in a lethal carcinoma of young people. Binds to papillomavirus E2 protein and is required for its transforming ability. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, atypical; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; BRD family

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: nucleoplasm; transcription elongation factor complex b; condensed nuclear chromosome; nuclear chromatin; cytoplasm; chromosome; nucleus

Molecular Function: protein binding; DNA binding; p53 binding; chromatin binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; transcription, DNA-dependent; inner cell mass cell proliferation; protein amino acid phosphorylation; chromosome segregation; chromatin remodeling; G1/S-specific transcription in mitotic cell cycle; positive regulation of RNA elongation from RNA polymerase II promoter; regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus; positive regulation of DNA binding
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