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anti-BTK antibody :: anti-Human, Mouse BTK Antibody

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Catalog # MBS852725
Unit / Price
  0.1 mg  /  $215 +1 FREE 8GB USB
Product Name

BTK, Antibody

Popular Item
Also Known As

BTK (Ab-222) Antibody

Product Synonym Names
AGMX1; ATK; Agammaglobulinaemia tyrosine kinase; B cell progenitor kinase; BPK; Bruton's tyrosine kinase; EC 2.7.10.2; Kinase EMB; kinase Btk
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
659
OMIM
300300
3D Structure
ModBase 3D Structure for Q06187
Species Reactivity
Human, Mouse
Specificity
BTK (Ab-222) antibody detects endogenous levels of total BTK protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human BTK around the phosphorylation site of tyrosine 222 (A-L-YP-D-Y).
Preparation and Storage
Store at-20 degree C for 1 year
Other Notes
Small volumes of anti-BTK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-BTK antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-BTK antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000

Immunohistochemistry (IHC) of anti-BTK antibody
Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BTK (Ab-222) antibody.
anti-BTK antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-BTK antibody
Western blot analysis of extracts from Hela cells, using BTK (Ab-222) antibody.
anti-BTK antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for BTK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
79,937 Da
NCBI Official Full Name
tyrosine-protein kinase BTK isoform 1
NCBI Official Synonym Full Names
Bruton tyrosine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
  [Similar Products]
NCBI Protein Information
tyrosine-protein kinase BTK
UniProt Protein Name
Tyrosine-protein kinase BTK
UniProt Synonym Protein Names
Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
AGMX1; ATK; BPK; ATK; BPK  [Similar Products]
NCBI Summary for BTK
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]
UniProt Comments for BTK
Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.
Product References and Citations for anti-BTK antibody
Sulekha Verma, J. Biol. Chem., Feb 2001; 276: 4671-4676. Yan M. Li, Cancer Res., Nov 2003; 63: 7630-7633. Malti Nikrad, Mol. Cancer Ther., Mar 2005; 4: 443-449. Feng Dong, Infect. Immun., Mar 2005; 73: 1861-1864. Hiroo Ueno, Mol. Biol. Cell, Feb 2000; 11: 735. Hidenori Hase, J. Biol. Chem., Nov 2002; 277: 46950-46958. Yasuyuki Hirashima, J. Biol. Chem., Jul 2003; 278: 26793-26802. Mingzhong Yao, J. Neurosci., Feb 2007; 27: 1422-1433.

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