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BUB1B elisa kit :: Human budding uninhibited by benzimidazoles 1 homolog beta (yeast) ELISA Kit

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Catalog # MBS9329895
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
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BUB1B elisa kit
Product Name

budding uninhibited by benzimidazoles 1 homolog beta (yeast) (BUB1B), ELISA Kit

Popular Item
Also Known As

Human Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, BUB1B ELISA Kit

Product Synonym Names
Human Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B) ELISA kit; BUB1beta; BUBR1; Bub1A; MAD3L; SSK1; hBUBR1; BUB1 budding uninhibited by benzimidazoles 1 homolog beta; MAD3/BUB1-related protein kinase; budding uninhibited by benzimidazoles 1 beta; budding uninhibit; budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
602860
3D Structure
ModBase 3D Structure for O60566
Species Reactivity
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Samples
Undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Assay Type
Sandwich
Detection Range
0.625 ng/ml - 20 ng/ml.
Sensitivity
0.1 ng/ml.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BUB1B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forBUB1Bpurchase
MBS9329895 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the budding uninhibited by benzimidazoles 1 homolog beta (yeast) (BUB1B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BUB1B. The ELISA analytical biochemical technique of the MBS9329895 kit is based on BUB1B antibody-BUB1B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BUB1B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BUB1B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
BUB1B elisa kit
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, NOT for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of BUB1B (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS9329895. Click to request current manual
NCBI/Uniprot data below describe general gene information for BUB1B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
119,545 Da
NCBI Official Full Name
mitotic checkpoint serine/threonine-protein kinase BUB1 beta
NCBI Official Synonym Full Names
BUB1 mitotic checkpoint serine/threonine kinase B
NCBI Official Symbol
BUB1B  [Similar Products]
NCBI Official Synonym Symbols
MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta
  [Similar Products]
NCBI Protein Information
mitotic checkpoint serine/threonine-protein kinase BUB1 beta; mitotic checkpoint kinase MAD3L; MAD3/BUB1-related protein kinase; budding uninhibited by benzimidazoles 1 homolog beta
UniProt Protein Name
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
UniProt Synonym Protein Names
MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1
UniProt Gene Name
BUB1B  [Similar Products]
UniProt Synonym Gene Names
BUBR1; MAD3L; SSK1; hBUBR1  [Similar Products]
UniProt Entry Name
BUB1B_HUMAN
NCBI Summary for BUB1B
This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008]
UniProt Comments for BUB1B
BUB1B: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with CASC5. Induced during mitosis. Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. Kinase activity stimulated by CENPE. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor; EC 2.7.11.1; Kinase, protein; Other group; BUB family

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: kinetochore; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; microtubule organizing center; outer kinetochore of condensed chromosome; spindle midzone; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: cell proliferation; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; apoptosis; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; protein amino acid phosphorylation; mitotic metaphase/anaphase transition

Disease: Mosaic Variegated Aneuploidy Syndrome 1; Premature Chromatid Separation Trait
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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