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C-ERC elisa kit :: Human Mesothelin ELISA Kit

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Catalog # MBS764860
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Mesothelin (C-ERC), ELISA Kit

Popular Item
Also Known As

Human Mesothelin ELISA Kit

Product Synonym Names
C-ERC,Mesothelin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS764860 COA
Sequence Length
622
OMIM
601051
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of C-ERC. No significant cross-reactivity or interference between C-ERC and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
62.5-4000pg/ml
Sensitivity
<37.5pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CERC were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CERC were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of C-ERC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forC-ERCpurchase
MBS764860 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mesothelin (C-ERC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C-ERC. The ELISA analytical biochemical technique of the MBS764860 kit is based on C-ERC antibody-C-ERC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C-ERC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C-ERC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
C-ERC elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- C-ERC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- C-ERC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C-ERC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C-ERC can be calculated.

Typical Standard Curve/Testing Data of C-ERC elisa kit
C-ERC elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS764860. Click to request current manual
NCBI/Uniprot data below describe general gene information for C-ERC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
67,940 Da
NCBI Official Full Name
mesothelin isoform 1 preproprotein
NCBI Official Synonym Full Names
mesothelin
NCBI Official Symbol
NCBI Official Synonym Symbols
MPF; SMRP
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NCBI Protein Information
mesothelin
UniProt Protein Name
Mesothelin
UniProt Synonym Protein Names
CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
MPF; MPF  [Similar Products]
UniProt Entry Name
MSLN_HUMAN
NCBI Summary for C-ERC
This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
UniProt Comments for C-ERC
MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cell surface; extracellular space; Golgi apparatus; membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cell adhesion; cell-matrix adhesion; pancreas development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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