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C3 elisa kit :: Complement C3 ELISA Kit

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Catalog # MBS135930
Unit / Price
  1 Kit  /  $445 +1 FREE 8GB USB
  5 Kits  /  $1,850 +2 FREE 8GB USB
C3 elisa kit
Product Name

Complement C3, ELISA Kit

Popular Item
Full Product Name

Mouse Complement C3 ELISA kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS135930 COA
3D Structure
ModBase 3D Structure for P01027
Host
Mouse
Specificity
This assay recognizes natural mouse C3. Pooled normal plasma from human, pig, rabbit, sheep, canine, and horse were assayed and no significant crossreactivity was observed. Pooled normal plasma from rat and rabbit resulted in significant co lor development.
Form/Format
Complete kit
Samples
mouse plasma, serum, and cell culture samp,les. For research use only
Preparation and Storage
Recommended Storage: 4 degree C
Minimum Shelf Life: 12 months from manufacture, see label for expiration date
Shipping Condition: Cold pack
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of C3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forC3purchase
MBS135930 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mouse Complement C3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C3. The ELISA analytical biochemical technique of the MBS135930 kit is based on C3 antibody-C3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
C3 elisa kit
Intended Uses: This mouse complement component 3 (C3) total antigen assay is intended for the quantitative determination of total C3 antigen in mouse plasma, serum, and cell culture samp,les. For research use only.

Background: Complement C3, the most abundant serum complement component, is a disulfide-linked 185kDa 1,637 amino acid glycoprotein which supports the classical, alternative, and lectin pathways of complement activation [1]. C3 is proteolytically activated by C3- convertase to the anaphylatoxin C3a and the opsonizing agent C3b [2]. Serum concentrations of C3 are increased during acute and chronic inflammation suc~ as rheumatoid arthritis, and are decreased due to increased consumption or autoimmune disorders such as systemic lupus erythematosus [3].
Product Categories/Family for C3 elisa kit
Sample Manual Insert of MBS135930. Click to request current manual
NCBI/Uniprot data below describe general gene information for C3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
60,952 Da[Similar Products]
NCBI Official Full Name
complement C3
NCBI Official Synonym Full Names
complement component 3
NCBI Official Symbol
NCBI Official Synonym Symbols
ASP; Plp; HSE-MSF; AI255234
  [Similar Products]
NCBI Protein Information
complement C3; complement factor 3; complement component 3d; acylation stimulating protein
UniProt Protein Name
Complement C3
UniProt Synonym Protein Names
HSE-MSFCleaved into the following 12 chains:Complement C3 beta chain; C3-beta-c; C3bc; Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein; ASPAlternative name(s):C3adesArg
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
C3bc; ASP  [Similar Products]
UniProt Entry Name
CO3_MOUSE
NCBI Summary for C3
This gene encodes complement protein C3 which plays a central role in the classical, alternative and lectin activation pathways of the complement system. The encoded preproprotein undergoes a multi-step processing to generate various functional peptides. Mice deficient in the encoded protein fail to clear bacteria from the blood stream upon infection, display diminished airway hyperresponsiveness and lung eosinophilia upon allergen-induced pulmonary allergy, and develop severe lung injury after deposition of IgG immune complexes. Deficiency of the homolog of the encoded protein in humans was found to be associated with increased susceptibility to infections, age-related macular degeneration, and atypical hemolytic uremic syndrome. [provided by RefSeq, Mar 2015]
UniProt Comments for C3
C3: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Defects in C3 are the cause of complement component 3 deficiency (C3D). A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis. Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin- containing structure known as Bruch membrane. Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; endopeptidase inhibitor activity; cofactor binding; lipid binding; C5L2 anaphylatoxin chemotactic receptor binding

Biological Process: positive regulation of developmental growth; immune system process; complement activation, alternative pathway; fatty acid metabolic process; complement activation; positive regulation of angiogenesis; positive regulation of activation of membrane attack complex; positive regulation of phagocytosis; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of type IIa hypersensitivity; innate immune response; positive regulation of protein amino acid phosphorylation; lipid metabolic process; blood coagulation; inflammatory response; complement activation, classical pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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