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C3 native protein :: Complement C3 Native Protein

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Catalog # MBS355514
Unit / Price
  0.1 mg  /  $205 +1 FREE 8GB USB
  1 mg  /  $445 +1 FREE 8GB USB
C3 native protein
Product Name

Complement C3, Native Protein

Popular Item
Full Product Name

Complement C3 Protein (Active)

Product Synonym Names
Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3, CPAMD1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS355514 COA
MBS355514 Technical Datasheet
Chromosome Location
Chromosome: 19; NC_000019.9 (6677846..6720662, complement). Location: 19p13.3-p13.2
OMIM
120700
3D Structure
ModBase 3D Structure for P01024
Host
Human Plasma
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid; Each vial contains 10mM PBS (pH7.4)
Preparation and Storage
Shipping: Blue ice.
Upon receipt, aliquot and store at -20°C or -80°C for long term.
Avoid repeated freeze and thaw cycles.
Other Notes
Small volumes of C3 native protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
C3 native protein
Purified human Complement C3 protein

Background: Complement component 3, often simply called C3, is a member of the complement system. The complement system is an important mediator of natural and acquired immunity. It consists of approximately 30 proteins that can exhibit catalytic activity, function as regulators, or act as cellular surface receptors. These components normally circulate in inactive forms and are activated by the classical, alternative, or lectin pathways. Complement component 3 plays a central role in all 3 activation pathways. People with C3 deficiency are susceptible to bacterial infection. Clinically, the Level of C3 in the blood may be measured to support or refute a particular medical diagnosis. For example, low C3 levels are associated with some types of kidney disease such as post-infectious glomerulonephritis and shunt nephritis.
Applications Tested/Suitable for C3 native protein
ELISA (EIA)
Application Notes for C3 native protein
Other applications have not been tested. The optimal dilutions should be determined by end user.
NCBI/Uniprot data below describe general gene information for C3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
complement C3
NCBI Official Synonym Full Names
complement component 3
NCBI Official Symbol
NCBI Official Synonym Symbols
ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1
  [Similar Products]
NCBI Protein Information
complement C3; prepro-C3; C3a anaphylatoxin; complement component C3; complement component C3a; complement component C3b; acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
UniProt Protein Name
Complement C3
UniProt Synonym Protein Names
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1Cleaved into the following 11 chains:Complement C3 beta chainComplement C3 alpha chainC3a anaphylatoxinAcylation stimulating protein; ASP; Alternative name(s):; C3adesArg
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CPAMD1; ASP  [Similar Products]
UniProt Entry Name
CO3_HUMAN
NCBI Summary for C3
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq, Feb 2009]
UniProt Comments for C3
Function: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Ref.5 Ref.11 Ref.14 Ref.15 Ref.16 Ref.22 Ref.25 Ref.29Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. Ref.5 Ref.11 Ref.14 Ref.15 Ref.16 Ref.22 Ref.25 Ref.29Acylation stimulating protein (ASP): adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2. Ref.5 Ref.11 Ref.14 Ref.15 Ref.16 Ref.22 Ref.25 Ref.29

Subunit structure: C3 precursor is first processed by the removal of 4 Arg residues, forming two chains, beta and alpha, linked by a disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylatoxin and generating C3b (beta chain + alpha' chain). C3dg interacts with CR2 (via the N-terminal Sushi domains 1 and 2). During pregnancy, C3dg exists as a complex (probably a 2:2:2 heterohexamer) with AGT and the proform of PRG2. Interacts with VSIG4. C3b interacts with herpes simplex virus 1 (HHV-1) and herpes simplex virus 2 (HHV-2) envelope glycoprotein C; this interaction inhibits the activation of the complement system. Interacts with S.aureus immunoglobulin-binding protein sbi, this prevents interaction between C3dg and CR2. Interacts with S.aureus fib. Interacts (both C3a and ASP) with C5AR2; the interaction occurs with higher affinity for ASP, enhancing the phosphorylation and activation of C5AR2, recruitment of ARRB2 to the cell surface and endocytosis of GRP77. Ref.7 Ref.10 Ref.17 Ref.18 Ref.30 Ref.31 Ref.43 Ref.44

Subcellular location: Secreted.

Tissue specificity: Plasma. The acylation stimulating protein (ASP) is expressed in adiopocytes and released into the plasma during both the fasting and postprandial periods. Ref.15 Ref.22

Post-translational modification: C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by insulin and dietary chylomicrons.Phosphorylation sites are present in the extracellular medium.

Polymorphism: There are two alleles: C3S (C3 slow), the most common allele in all races and C3F (C3 fast), relatively frequent in Caucasians, less common in Black Americans, extremely rare in Orientals.

Involvement in disease: Complement component 3 deficiency (C3D) [MIM:613779]: A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.32 Ref.35 Ref.36 Ref.37 Ref.48 Ref.49Age-related macular degeneration 9 (ARMD9) [MIM:611378]: A form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.32 Ref.50Hemolytic uremic syndrome atypical 5 (AHUS5) [MIM:612925]: An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Other genes may play a role in modifying the phenotype. Ref.32 Ref.51 Ref.52Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage. Ref.32

Sequence similarities: Contains 1 anaphylatoxin-like domain.Contains 1 NTR domain.

Caution: According to Ref.44, the interaction surface between C3 and CR2 reported in Ref.36 is artifactual and can be ascribed to the presence of zinc acetate in the buffer.
Precautions
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Disclaimer
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