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C4b elisa kit :: Human Complement fragment 4b, C4b ELISA Kit

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Catalog # MBS700316
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Complement fragment 4b, C4b, ELISA Kit

Popular Item
Also Known As

Human Complement fragment 4b, C4b ELISA Kit

Product Synonym Names
Human Complement fragment 4b; C4b ELISA Kit; Complement fragment 4b; C4b
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS700316 Testing Data
Sequence Length
1744
OMIM
614379
3D Structure
ModBase 3D Structure for P0C0L5
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human C4b. No significant cross-reactivity or interference between human C4b and analogues was observed.
Samples
Serum, plasma
Assay Type
Sandwich
Detection Range
15.6 ng/ml -1000 ng/ml.
Sensitivity
The minimum detectable dose of human C4b is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of C4b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forC4bpurchase
MBS700316 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement fragment 4b, C4b, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C4b. The ELISA analytical biochemical technique of the MBS700316 kit is based on C4b antibody-C4b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C4b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C4b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
C4b elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C4b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C4b present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C4b is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4b bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of C4b elisa kit
C4b elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS700316. Click to request current manual
NCBI/Uniprot data below describe general gene information for C4b. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
192,751 Da
NCBI Official Full Name
complement C4-B preproprotein
NCBI Official Synonym Full Names
complement component 4B (Chido blood group)
NCBI Official Symbol
NCBI Official Synonym Symbols
CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
  [Similar Products]
NCBI Protein Information
complement C4-B; Chido form of C4; complement C4B1a; basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
UniProt Protein Name
Complement C4-B
UniProt Synonym Protein Names
Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3Cleaved into the following 6 chains:Complement C4 beta chain; Complement C4-B alpha chain; C4a anaphylatoxin; C4b-B; C4d-B; Complement C4 gamma chain
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CO4; CPAMD3
C4B_2  [Similar Products]
UniProt Entry Name
CO4B_HUMAN
NCBI Summary for C4b
This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9. [provided by RefSeq, Jul 2008]
UniProt Comments for C4b
Function: Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.2 PublicationsManual assertion based on experiment in:Ref.16
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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