• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CA2 elisa kit :: Mouse CA2 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2513124
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CA2, ELISA Kit

Popular Item
Also Known As

Mouse CA2 (Carbonic Anhydrase 2) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
260
OMIM
259730
3D Structure
ModBase 3D Structure for P00918
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CA2. No significant cross-reactivity or interference between Mouse CA2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CA2 and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
1.563-100ng/mL
Sensitivity
Min: 0.938ng/mL; Max: 100ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCA2purchase
MBS2513124 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CA2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CA2. The ELISA analytical biochemical technique of the MBS2513124 kit is based on CA2 antibody-CA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CA2 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CA2 in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CA2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CA2. You can calculate the concentration of Mouse CA2 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CA2 elisa kit
CA2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2513124. Click to request current manual
NCBI/Uniprot data below describe general gene information for CA2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,246 Da
NCBI Official Full Name
CA2, partial
NCBI Official Synonym Full Names
carbonic anhydrase II
NCBI Official Symbol
NCBI Official Synonym Symbols
CAC; CAII; Car2; CA-II; HEL-76
  [Similar Products]
NCBI Protein Information
carbonic anhydrase 2; carbonate dehydratase II; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; epididymis luminal protein 76
UniProt Protein Name
Carbonic anhydrase 2
UniProt Synonym Protein Names
Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CAC; CA-II  [Similar Products]
UniProt Entry Name
CAH2_HUMAN
NCBI Summary for CA2
The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
UniProt Comments for CA2
CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Lyase; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: extracellular space; microvillus; apical part of cell; basolateral plasma membrane; axon; cytoplasm; plasma membrane; cytosol; myelin sheath

Molecular Function: protein binding; carbonate dehydratase activity; zinc ion binding

Biological Process: secretion; positive regulation of osteoclast differentiation; positive regulation of cellular pH reduction; positive regulation of synaptic transmission, GABAergic; one-carbon compound metabolic process; odontogenesis of dentine-containing teeth; bicarbonate transport; response to estrogen stimulus; response to zinc ion; regulation of intracellular pH; morphogenesis of an epithelium; positive regulation of bone resorption; kidney development; response to pH

Disease: Osteopetrosis, Autosomal Recessive 3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000