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CA9 elisa kit :: Human CA9 ELISA Kit

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Catalog # MBS2502398
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CA9, ELISA Kit

Popular Item
Full Product Name

Human CA9 (Carbonic Anhydrase IX) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2502398 COA
OMIM
603179
3D Structure
ModBase 3D Structure for Q16790
Species Reactivity
Specificity
This kit recognizes natural and recombinant Human CA9. No significant cross-reactivity or interference between Human CA9 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Intended Uses
This ELISA kit applies to the in vitro quantitative determination of Human CA9 concentrations in serum, plasma and other biological fluids.
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CA9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCA9purchase
MBS2502398 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CA9, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CA9. The ELISA analytical biochemical technique of the MBS2502398 kit is based on CA9 antibody-CA9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CA9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CA9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CA9 elisa kit
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CA9. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CA9 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CA9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CA9. You can calculate the concentration of CA9 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CA9 elisa kit
CA9 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2502398. Click to request current manual
NCBI/Uniprot data below describe general gene information for CA9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49,698 Da
NCBI Official Full Name
carbonic anhydrase 9
NCBI Official Synonym Full Names
carbonic anhydrase IX
NCBI Official Symbol
NCBI Official Synonym Symbols
MN; CAIX
  [Similar Products]
NCBI Protein Information
carbonic anhydrase 9; CA-IX; P54/58N; RCC-associated antigen G250; RCC-associated protein G250; carbonate dehydratase IX; carbonic dehydratase; membrane antigen MN; pMW1; renal cell carcinoma-associated antigen G250
UniProt Protein Name
Carbonic anhydrase 9
UniProt Synonym Protein Names
Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
G250; MN; CA-IX; CAIX; RCC-associated antigen G250  [Similar Products]
UniProt Entry Name
CAH9_HUMAN
NCBI Summary for CA9
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
UniProt Comments for CA9
CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; Nucleolus; Energy Metabolism - nitrogen; EC 4.2.1.1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: basolateral plasma membrane; microvillus membrane; nucleolus; integral to membrane; plasma membrane

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: response to drug; secretion; bicarbonate transport; morphogenesis of an epithelium; one-carbon compound metabolic process; response to testosterone stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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