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CA9 elisa kit :: Mouse carbonic anhydrase IX ELISA Kit

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Catalog # MBS939749
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
CA9 elisa kit
Product Name

carbonic anhydrase IX (CA9), ELISA Kit

Popular Item
Also Known As

Mouse Carbonic anhydrase 9, CA9 ELISA Kit

Product Synonym Names
Mouse Carbonic anhydrase 9 (CA9) ELISA kit; CAIX; MN; RCC-associated protein G250; carbonic dehydratase; carbonic anhydrase IX
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
437
3D Structure
ModBase 3D Structure for Q8VHB5
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse CA9. No significant cross-reactivity or interference between mouse CA9 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
0.156 ng/ml -10 ng/ml
Sensitivity
Typically less than 0.039 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CA9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCA9purchase
MBS939749 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the carbonic anhydrase IX (CA9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CA9. The ELISA analytical biochemical technique of the MBS939749 kit is based on CA9 antibody-CA9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CA9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CA9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CA9 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CA9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CA9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CA9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS939749. Click to request current manual
NCBI/Uniprot data below describe general gene information for CA9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47,265 Da
NCBI Official Full Name
carbonic anhydrase 9
NCBI Official Synonym Full Names
carbonic anhydrase 9
NCBI Official Symbol
NCBI Official Synonym Symbols
Ca9; CAIX; MN/CA9
  [Similar Products]
NCBI Protein Information
carbonic anhydrase 9; CA-IX; carbonic anhydrase IX; carbonate dehydratase IX; membrane antigen MN homolog
UniProt Protein Name
Carbonic anhydrase 9
UniProt Synonym Protein Names
Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN homolog
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Car9; CA-IX; CAIX  [Similar Products]
UniProt Entry Name
CAH9_MOUSE
UniProt Comments for CA9
CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family.

Protein type: Energy Metabolism - nitrogen; Nucleolus; Membrane protein, integral; Lyase; EC 4.2.1.1

Cellular Component: cell projection; membrane; basolateral plasma membrane; integral to membrane; plasma membrane

Molecular Function: carbonate dehydratase activity; zinc ion binding; metal ion binding; lyase activity

Biological Process: secretion; morphogenesis of an epithelium; one-carbon compound metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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