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CACYBP elisa kit :: Mouse CACYBP ELISA Kit

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Catalog # MBS2508565
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CACYBP, ELISA Kit

Popular Item
Also Known As

Mouse CACYBP (Calcyclin Binding Protein) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
185
OMIM
606186
3D Structure
ModBase 3D Structure for Q9HB71
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CACYBP. No significant cross-reactivity or interference between Mouse CACYBP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CACYBP and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CACYBP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCACYBPpurchase
MBS2508565 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CACYBP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CACYBP. The ELISA analytical biochemical technique of the MBS2508565 kit is based on CACYBP antibody-CACYBP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CACYBP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CACYBP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CACYBP elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CACYBP in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CACYBP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CACYBP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CACYBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CACYBP. You can calculate the concentration of Mouse CACYBP in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CACYBP elisa kit
CACYBP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2508565. Click to request current manual
NCBI/Uniprot data below describe general gene information for CACYBP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,228 Da
NCBI Official Full Name
calcyclin-binding protein isoform 2
NCBI Official Synonym Full Names
calcyclin binding protein
NCBI Official Symbol
CACYBP  [Similar Products]
NCBI Official Synonym Symbols
SIP; GIG5; PNAS-107; S100A6BP
  [Similar Products]
NCBI Protein Information
calcyclin-binding protein; S100A6-binding protein; Siah-interacting protein (SIP); growth-inhibiting gene 5 protein; hCacyBP
UniProt Protein Name
Calcyclin-binding protein
UniProt Synonym Protein Names
S100A6-binding protein; Siah-interacting protein
Protein Family
UniProt Gene Name
CACYBP  [Similar Products]
UniProt Synonym Gene Names
S100A6BP; SIP; CacyBP; hCacyBP  [Similar Products]
UniProt Entry Name
CYBP_HUMAN
NCBI Summary for CACYBP
The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for CACYBP
CACYBP: a nuclear/cytoplasmic protein involved in calcium-dependent ubiquitination and proteosomal degradation of target proteins. A possible molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). Interacts with proteins of the S100 family at physiological calcium concentrations. Component of some large E3 complexes and interacts directly with SIAH1, SIAH2 and SKP1A. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated. Two alternatively spliced isoforms have been described.

Protein type: Ubiquitin conjugating system; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q24-q25

Cellular Component: nucleoplasm; neuron projection; nuclear envelope lumen; cytoplasm; beta-catenin destruction complex

Molecular Function: protein binding; protein homodimerization activity

Biological Process: cardiac muscle cell differentiation; positive regulation of DNA replication; aging
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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